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Cell Journal، جلد ۱۳، شماره Supplement، صفحات ۰-۰
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عنوان انگلیسی P-6: Neurogenic Induction in Human Adipose Derived Stem Cells
چکیده انگلیسی مقاله Objective: Multipotent stem cells have been detected in some tissues in the adult, participating in normal replacement and repair. There are some evidences to suggest that adipose stromal cells (ADSCs) not only differentiated into mesodermal cells, but also accept the fate of endodermal and ectodermal cell types. ADSCs can be expanded rapidly in vitro and can be obtained by a less invasive method. In this study, we attempted to evaluation the protocol for the induction of ADSCs into neurons. Materials and Methods: ADSCs was isolated and characterized a population of human primate adipose tissue stromal cells containing multipotent progenitor cells. These cells were induced to differentiate into neural-like cells and analyzed for expression of the neural specific markers. Differentiated ADSCs were assessed for Nestin, MAP2 and GFAP expression by immunocytochemistry and semi quantitative RT-PCR techniques. Moreover, MTT assay was applied to detect cell viability. Results: Immunocytochemical analysis demonstrated that ADSCs had large expression of the neural-specific markers following differentiation. Additionally, soon after differentiation, this protocol showed the highest percentage of mature neural-like cells, however with passing time, the neural like state was transient and reversible. MTT assay showed that induction agents led to more cell death. Conclusion: Analysis showed that neural-like cell differentiation potential of ADSCs in chemical induction was rapidly but transitory.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/1538
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