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Cell Journal، جلد ۱۳، شماره Supplement، صفحات ۰-۰
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عنوان انگلیسی P-24: Study of Methylation Status of CpG Islands Located in Mir-9 Family Members Chromosomal Regions in Human Gastric Adenocarcinoma
چکیده انگلیسی مقاله Objective: MicroRNAs (miRNAs) are small single stranded noncoding RNAs which regulate target gene expression through pairing with target mRNAs, inducing direct mRNA degradation or translational inhibition. DNA hypermethylation in the miRNA 5' regulatory region can account for the downregulation of miRNA in tumors. Hsa-mir-9 family has three members transcribing from three loci including hsamir- 9-1(1q22), hsa-mir-9-2 (5q14.3), and hsa- mir- 9-3 (15q26.1). All of the loci have overlap with CpG islands. DNA methylation associated silencing of mir-9 is atttributed to the development of some human cancer metastasis. Decreasing expression of mir-9 is reported in human gastric adenocarcinoma and NFκB1 is assigned as its target mRNA. Materials and Methods: Using methylation-specific PCR, we studied the methylation status of CpG islands located in mir 9-1, mir-9-2 and mir 9-3 chromosomal regions in human gastric adenocarcinoma AGS cell line, 30 human gastric adenocarcinoma samples and 30 matched normal gastric samples. Results: In AGS cell line mir 9-1 and 9-2 loci CpG islands were methylated and mir9-3 showed heterogeneous methylation status but in gastric adenocarcinoma samples mir9-1 showed stochastic methylation status and there was no meaningful difference between tumoral and normal samples (p=0.07). Mir-9-2 locus CpG island was unmethylated in all of tumoral and marginal samples however mir9-3 CpG island had heterogeneous methylation status in all of samples. Conclusion: We showed that methylation of these CpG islands were not meaningfully different between normal and tumoral gastric adenocarcinoma specimens and cannot regulate mir-9 expression in this type of gastric cancer
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/1556
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