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Cell Journal، جلد ۱۳، شماره Supplement، صفحات ۰-۰
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عنوان انگلیسی P-31: Colorimetric Detection and Quantification of Small Anti-Cancer Drug Molecules Based on G-Quadruplex Binding, Using Unmodified Gold Nanoparticles
چکیده انگلیسی مقاله Objective: Stabilizing G-quadruplexes as regulatory structures in telomere DNA and promoter regions by intercalator ligands provide a new route to anti-cancer therapies. The purpose of this study was to test the hypothesis that an array of unmodified gold nanoparticles and various DNA types and conformations could provide qualitative and quantitative information about intercalator anticancer ligands. Materials and Methods: Our model system included two single-stranded DNA (ss-DNA) sequences: A 22- mer G-rich DNA (G-DNA) which carries a piece of the human telomeric sequence, and its complementary sequence (C-DNA). As controls, a randomly synthesized ss-DNA (R-DNA) and Calf-Thymus (genomic) from Sigma were used. The conformation of the model oligonucleotides was characterized by Circular dichroism (CD) spectroscopy. Gold nanoparticle (AuNP) synthesis was done by citrate reduction of HAuCl4. For colorimetric analysis, digital photographs of the array were taken and analyzed by MATLAB software for further quantification using mean Red- Blue-Green (RGBs). Fluorescence and UV-Visible Spectroscopy were used as extra informative tools. Results: The array system was designed and optimized with unmodified gold nanoparticles and DNA in different ionic strengths (salt concentrations) and ligand concentrations. The molecular basis of this array system is the electrostatic absorption of ss-DNA on Au-NP surface which delays salt-induced Au-NP aggregation. The desorption of ss-DNA by ligand intercalation occurs and a gradual red to blue color change is seen. By this simple gradual color change, ligands could be detected and their affinity to intercalate in G-DNA determined. Conclusion: An array system has been designed based on unmodified gold nanoparticles for the first time; which is simple, rapid, requiring no complicated apparatus and providing qualitative and useful quantitative information about small anti-cancer drug molecules.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/1563
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