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Cell Journal، جلد ۱۳، شماره Supplement، صفحات ۰-۰
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عنوان انگلیسی P-39: The Chemikine Receptor RDC-1 is Marker for Alveolar Rhabdomyosarcoma
چکیده انگلیسی مقاله Objective: Stromal derived factor-1 (SDF-1, a member of the alpha chemokines (CXC) and ligand for the Cell Journal(Yakhteh), Vol 13, Suppl 2, Spring 2011 42 Abstracts of the 2nd National and 1st Int. Congress on "Cellular and Molecular Advances in Non-Contagious Diseases" CXCR4 receptor, has been shown to be an effective chemoattractant for various CXCR4-expressing cells. SDF-1 is important for metastasis of cancer cells expressing its receptor. Rhabdomyosarcoma (RMS) frequently infiltrates bone marrow and it was previously reported that SDF-1/CXCR4 axis plays a role in migration of RMS cell lines. Recent studies have shown that RDC-1 is expressed on prostate cancers and is positively correlated with the stage of disease. This prompted us to examine the expression of CXCR4 and RDC-1 in RMS cells lines and tissue samples from patients diagnosed with RMS using RT-PCR, Flow Cytometry and Immunohistochemistry. Materials and Methods: Our data show that RDC-1 is highly expressed in Alveolar (A-RMS) cell lines, but not in embryonal (E-RMS). In contrast, CXCR4 is expressed in both A-RMS and E-ARMS cell lines. Moreover, we found that RDC-1 is involved in SDF- 1-induced cell proliferation of A-RMS cells. Next, we examined the expression of CXCR4 and RDC-1 on tissue blocks by imminuhistochemistry and found that CXCR4 is expressed on both A-RMS and E-RMS subtypes. In contrast, RDC-1 is mostly expressed on A-RMS. As the majority of A-RMS cells harbour the translocation t(2;13)(35q;14q) resulting in a PAX3- FKHR fusion gene, we trasnfected PAX3-FKHR gene in E-AMS (RD cell line) and found that RDC-1 is highly expressed in RD-PAX-FKHR cells, but not in RD-wt. Conclusion: We present evidence for the first time that the RDC-1 is highly expressed on A-RMS samples and may thus be a suitable marker for diagnosis and a potential target for treatment of A-RMS.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/1571
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