این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۳، شماره Supplement، صفحات ۰-۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی P-42: Dedifferentiation of NIH3T3 Cells in the Presence and Absence of 5-Aza-2'Deoxycytidine and Trichostatin A by Cell-FreeExtract of Embryonic Stem cells
چکیده انگلیسی مقاله Objective: Nowadays, cell reprogramming is very fashionable in regenerative medicine. Therefore scientists try to do reprogramming in more efficient ways. There is evidence to show that cell reprogramming is controlled by epigenetic function, so cleaning of epigenetic may help to reprogramming. 5-Aza-2'- deoxycytidine (5-Aza-dc, methyltransferase inhibitor) and Trichostatin A (TSA, Histone deacetylase inhibitor) can partially clear epigenetic. Materials and Methods: In the present study, we de-differentiated NIH3T3 cells in the presence and absence of 5-Aza-dc and TSA by mouse Embryonic Stem Cell (mESC) extract. Then we checked cells morphology and alkaline phosphatase expression in the reprogrammed cells. Results: The cells without treatment showed morphologic changes in the day after reprogramming and formed colonies on the second day. Growth of these cells that were passaged every two days was similar to mESCs due to formation of embryoid like bodies. In pre-treated cells, colonies were formed four days after reprogramming and the number of colonies was remarkably less than the untreated cells. NIH3T3 cells reprogrammed by mESC extract showed alkaline phosphatase activity while NIH3T3 cells that were both treated with epigenetic eraser and reprogrammed by ESC extract didn’t show alkaline phosphatase expression. Conclusion: According to these data efficiency of dedifferentiation is better by using mESC extract and without epigenetic eraser materials.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/1574
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات