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Cell Journal، جلد ۱۳، شماره Supplement، صفحات ۰-۰
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عنوان انگلیسی P-55: Gene Expression of α2β1, α4β1 and α5β1Integrins: Possible Indicators in the Metastatic Behavior of Prostate Cancer Cell Lines
چکیده انگلیسی مقاله Objective: Integrins, which are transmembrane receptors for extracellular matrix proteins, play a key role in cell survival, proliferation, migration, gene expression and activation of growth factor receptors. Their functions and expression are deregulated in several types of cancer. In prostate cancer, tumor cells express an abnormal integrin repertoire and are surrounded by a markedly different ECM as compared to normal prostate. We review in this article the role of integrins in prostate cancer progression and their potential as therapeutic target. Materials and Methods: Prostate cancer cells, PC3 and DU145, adhesion and inhibition assays using anti-β1 integrin antibody were investigated. Immunofluorescence microscopy and Focal adhesion assays were performed using monoclonal primary antihuman vinculin (h-Vin 1, 1: 400 dilution) or mouse anti human integrin antibodies and goat anti mouse-IgG fluorescein isothiocyanate (FITC, 1: 500 dilution) as a secondary antibody. RNA extraction from prostate cancer cell lines was done for gene expression study. The expression level of integrin subunits in cancer prostate cell lines was evaluated by semi-quantitative RT-PCR and FACS analysis. Results: DU145 and PC3 cell attachment on CollagenI was inhibited by anti-β1 integrin antibody by approximately 97.3% and 95% respectively and the same assay with these two cell lines on fibronectin was 67.4% and 43.2% respectively. The results suggested that the anti-β1 integrin antibody inhibited the attachment of DU145 and PC3 cells to FN-coated plates. Gene expression study revealed that DU145 in contrast to PC3 did not express α4 subunit of integrin while both cell lines expressed α5, α2 and β1 integrin subunits. Conclusion: Our results show that one of the mechanisms involved in cell adhesion changes in the two cell lines is the level of α4 gene expression. It should be noted that DU145 cell line metastasizes to brain while PC3 cell line is responsible for the metastasis to bone. Therefore we conclude that the variability in α4 gene expression may be a causative factor in the difference observed in the metastatic process.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/1587
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