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Cell Journal، جلد ۱۳، شماره Supplement، صفحات ۰-۰
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عنوان انگلیسی I-3: Rose Bengal Acetate PhotodynamicTherapy Induces Apoptosis and Autophagy of oHeLa Cells
چکیده انگلیسی مقاله Photodynamic therapy (PDT) is an approved anticancer therapy that kills cancer cells by the photochemical generation of ROS following absorption of visible light by a photosensitizer, selectively accumulated in tumours. Cell deaths in PDT occur by the efficient induction of apoptosis, necrosis and autophagy, or by a combination of the three mechanisms. However, the exact pathways of apoptotic induction and the role of autophagy in PDT are still a matter of debate. To this purpose, we investigated the induction of both apoptosis and autophagy in HeLa, after incubation of cells with RBAc (10-5 M) and irradiation (1.6 J/cm2 as total light dose). RBAc-PDT gave rise to 40% of apoptotic cells and to 25% of autophagy at 4-12hours and 8hours after irradiation respectively. Western blots of active caspases and the release of cytochrome c suggest a primary involvement of the intrinsic apoptotic pathway (1-4hours post-PDT) followed by the activation of the extrinsic and the caspase 12 dependent pathways (from 18hours post-PDT). Since, the pan-caspases inhibitor zVAD was not able to completely prevent cell deaths, autophagy was detected by biochemical (Western blot of LC3B protein) and morphological criteria (TEM, cytochemistry). The simultaneous onset of apoptosis and autophagy following RBAc-PDT is of remarkable interest in consideration of the findings that autophagy can result in class II presentation of antigens and thus explain why low dose PDT can yield anti-tumour vaccines.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/1486
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