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Cell Journal، جلد ۱۳، شماره Supplement، صفحات ۰-۰
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عنوان انگلیسی I-8: Immune Porperties and Regenerative Potential of Human Mesenchymal Stem Cells Derived from Umbilical Cord Tissue
چکیده انگلیسی مقاله Objective: Mesenchymal stem cells (MSC) are adherent fibroblast-like cells with multipotent properties. In addition to bone marrow and fat tissue, they can easily be isolated from the Wharton´s jelly region of umbilical cords (UCMSC). We investigated the immunosuppressive potential, the endothelial and cardiovascular differentiation capacity of UCMSC, the seeding feasibility on synthetic scaffolds as well as their ability to grow under GMP-compliant culture conditions. In addition, it was investigated whether UCMSC influence the phenotype of human cardiac stem cells (CSC). Materials and Methods: UCMSC were isolated as previously described1, cultured in GMP-compliant xeno- and serum-free media and tested for their immunosuppressive properties in CSFE assays with allogeneic mixed lymphocyte reactions (MLR). Endothelial and cardiomyocyte differentiation was driven by incubating the cells in vascular growth factors and oxytocin, respectively. Cells were also seeded on Titanium-coated expanded polytetrafluorethylene (Ti-ePTFE) scaffolds and analyzed for viability, proliferation, and morphology under static and dynamic conditions in a self-made bioreactor, respectively. CSC were derived from fresh heart explant cultures after cardiac transplantation. Results: The cardiomyocyte phenotype of differentiated UCMSC was verified morphologically and with an optimized set of cardio markers. Endothelial-like UCMSC expressed von Willebrandt factor and established network structures in Matrigel™. UCMSC effectively suppressed MLR via expression of prostaglandin E2. Furthermore, UCMSC can be seeded on Ti-ePTFE scaffolds and maintain their viability and morphology. In addition, conditioned medium of UCMSC promote maturation of CSC into cardiomyocytes. Finally, GMPcompliant growth media qualify for long-term cultures and expansion of UCMSC. Conclusion: The umbilical cord tissue is an easily accessible source for mesenchymal stem cells. Due to their potential to give rise to cardiovascular cells and the vasculature as well as due to their immunosuppressive properties UCMSC constitute an attractive tool in regenerative and transplant medicine. UCMSC might be used undifferentiated to trigger endogenous repair by CSC or can be used as cardio-differentiated cells on biocompatible scaffolds for tissue engineering
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/1491
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