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Cell Journal، جلد ۱۳، شماره Supplement، صفحات ۰-۰
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عنوان انگلیسی O-10: Reprogramming of Fibroblast Cells toward Embryonic Stem Cells and Differentiation to Adipocytes
چکیده انگلیسی مقاله Objective: The purpose of regenerative medicine is to generate pluripotent cells with the genome of the patient and induce differentiation in these cells for transplantation. Stem cell therapy using induced Pluripotent Stem (iPS) cells provides hope for the treatment of diseases in the future. Several methods have been investigated to induce reprogramming of somatic cells. Using cell extract for reprogramming, seems to be very efficient and results in get the large quantities of reprogrammed cells. Materials and Methods: In this study we reprogrammed NIH3T3 cells to mouse Embryonic Stem Cells (mESCs) by mESC extract. Reprogrammed cells formed colonies and could form embryoid bodies. Expression of stem cell specific genes was corroborated by alkaline phosphatase assay, immunoflurescence staining for Nanog and Sox2 and also RT-PCR for Oct3/4 in colonies. In order to clarify the differentiation property of the reprogrammed cells, the cells were cultured in adipogenic media and then the differentiated cells were stained with oil red. Results: The reprogrammed cells showed lipid droplets in their cytoplasm after exposure to adipogenic media. droplets of the lipids stained red that indicated cell differentiation towards adipocytes. Conclusion: These data provide evidence for the generation of ES like cells from differentiated somatic cells by mESC extract and the differentiation capacity of these cells.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/1518
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