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Cell Journal، جلد ۱۳، شماره Supplement، صفحات ۰-۰
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عنوان انگلیسی O-18: Transplantation of Human Umbilical Cord Blood Derived Mononuclear Cells Normalize Gelycemia
چکیده انگلیسی مقاله Objective: Hyperglycemia is the major health problem for the diabetic subjects. According nto the recent work umbilical cord blood contains circulating stem or progenitor cells. The present study explored whether human umbilical cord blood (HUCB) derived mononuclear cells could improve hyperglycemia in Rats. Materials and Methods: Blood from umbilical cord (n=10) was obtained from department of gynecology and obstetrics Babol university school of medicine after receiving the mothers informed consent. Immediately after collection of UCB samples, approximately 10 mL of collected cord blood was mixed with a same volume of phosphate-buffered saline (PBS). Ten ml of this cell suspension was carefully layered over 10 ml of Ficollpaque TM premium. After the viability and counting were determined, the mononuclear cells were centrifuged for 15 min at 1800 rpm, then 250 μl of PBS solution was added for final dilution. On the day of injection, cells were washed in phosphate - buffered saline. Cell viability was determined by 0.4% trypan blue dye staining before infusion. The serum glucose was measured before the study groups received the MNCs and at 1, 4, 7, 12 and 15 weeks following the treatment. Results: Ten cord blood samples were used for mononuclear cell separation. The mean collection volume was 41-52 ml, containing between 16×106 and 34.8×106 cells /250μl by manual counting after Ficoll centrifugation and washing (mean 23.56×106 cells /250μl). Cell viability ranged between 78-96% (mean 92 ± 8%). Approximately 23.56×106 cells were transplanted .Our results indicate that blood glucose levels within a week after MNC administration, were significantly reduced (245.74 ± 2.37 mg/dl and decreased until reaching almost normal glycemic values 15 weeks later (108.26 ± 6.84 mg/dl). Conclusion: Our results showed that glucose levels in MNCs implanted groups began to decrease. We found that MNCs can correct glycemia in hyperglycemic models. Moreover, in future the use of human umbilical cord blood derived mononuclear cells could opens new possibilities for the treatment of diabetic patients.
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/1526
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