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Cell Journal، جلد ۱۳، شماره Supplement، صفحات ۰-۰
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عنوان انگلیسی O-20: Repair of Spinal Cord Injury (SCI) Using Monocytes Transfected with Adenoviral Vector Expressing Decorin in a Rat SCI model
چکیده انگلیسی مقاله Objective: Subsequent to spinal cord injury many pathological changes may occur that lead to inappropriate environment for repair, most important of which are cyst formation, glial scar and ineffectiveness of monocytes. Although monocytes have restorative roles in tissue lesions, but with the incidence of spinal injury, monocyte responses in the lesion site are inadequate and their numbers are less than expected. The glial scar resulted from SCI is rich of chondroitin sulfate proteoglycans (CSPG) that inhibit growth of the axons. Decorin is a small leucine rich proteoglycan that inhibits CSPGs through various mechanisms. Decorin not only directly promotes growth of neurons, but also inhibits glial scar formation. In this study we tried to improve treatment results using a combination of monocyte and decorin features. Materials and Methods: Rats were divided into four groups of six at random. Spinal cord injury was then performed under general anaesthesia using the weight dropping method. The cells were injected at 4 days postspinal cord injury. Group one included rats receiving normal saline, group two received monocytes, group three received monocytes infected with adenoviruses encoding the beta-galactosidase gene, and group four received monocytes infected with adenoviruses encoding the decorin gene. A Basso, Beattie and Bresnahan (BBB) score test performed for four weeks. Two weeks before the end, biotin dextran amine was injected intra cerebrally and at the end of the fourth week tissue staining was performed. Results: There was a significant difference in BBB scores between groups one and four. There were no significant differences between other groups. There was a significant difference in axon staining results between two,three and four groups with group one, and also between four group with two and three groups.but was no significant difference between two group with three group. Conclusion: Combined use of these methods (Decorin expressing monocytes) had better results in comparison with monocytes alone. In this study the two methods were used simultaneously. The time of injection of monocytes is very important and so we suggest that other injection times be tested
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/1528
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