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Cell Journal، جلد ۱۳، شماره Supplement، صفحات ۰-۰
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عنوان انگلیسی O-24: In Vitro Response of Adipose Tissue-Derived Stem Cell to Differently Processed 316L Stainless Steel Surfaces
چکیده انگلیسی مقاله Objective: The need for rehabilitation of the damaged heart continues a formidable challenge over the years. To find the solution, researchers are working toward tissue engineering techniques to provide a repair strategy to regenerate damaged cardiac cells. Adipose tissuederived stem cells (ADSCs) are one of the most fundamental cell types of mesenchymal origin, so it can be taken as a model to investigate the behavior of other cells of the same origin. The present work assesses ADSCs adhesion on steel alloy substrates to improve cellsubstrate interaction. Materials and Methods: Cell culture experiments conducted using Balb/c mice adipose tissue derived-stem cells. After each time point, adherent cells counted with a hemacytometer. To investigate the morphological characteristics, three passaged ADSCs were grown on substrates for 3 days, and then observed under a SEM. For assessing cell differentiation, cells were stained by primary (cardiac muscle troponin I) and secondary (anti- mouse FITC-conjugated IgG antibody) antibodies. Results: The number of adherent ADSCs on rod substrates was higher than sheet samples. Cell proliferation was also significantly higher on rod samples after 3 and 5 days of culture. SEM results also confirmed spreading pattern being higher on rod substrate. By Immunofluorescence analysis, slight differences were observed between differentiated ADSCs on rod and sheet substrates. Conclusion: The skill to alter structure and molecular information with controlled manner at the biomaterial/ tissue interface may lend itself to engineering of biomaterial surfaces, which may be specifically suitable for conducting the differentiation of stem cells into definite lineage
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نشانی اینترنتی http://celljournal.org/journal/article/abstract/1532
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