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International Journal of Fertility and Sterility، جلد ۱۰، شماره ۳، صفحات ۳۱۰-۳۱۹
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عنوان انگلیسی Expression profile of developmentally important genes in pre- and peri-implantation goat embryos produced in vitro
چکیده انگلیسی مقاله Background: Little is understood about the regulation of gene expression during early goat embryo development. This study investigated the expression profile of 19 genes, known to be critical for early embryo development in mouse and human, at five different stages of goat in vitro embryo development (oocyte, 8-16 cell, morula, day-7 blastocyst, and day 14 blastocyst). Materials and methods: Stage-specific profiling using RT-qPCR revealed robust and dynamic patterns of stage-specific gene activity that fall into four major clusters depending on their respective mRNA profiles. Results: The gradual pattern of reduction in the maternally stored transcripts without renewal thereafter (cluster-1: LIFR1, BMPR1, ALK4, ID3, CTNNB, AKT, OCT4, REX1, ERK1 and SMAD1&5) implies that their protein products are essential during early cleavages when the goat embryo is silent and reliant to the maternal legacy of mRNA. The potential importance of transcription augment at day-3 (cluster-2: FZD, C-MYC, CDC25a, SOX2) or day-14 (cluster-3: FGFR4, NANOG) suggests that they are nascent embryonic mRNAs which intimately involved in the overriding of MET or regulation of blastocyst formation, respectively. The observation of two expression peaks at both day-3 and day-14 (cluster-4: GATA4, CDX2) would imply their potential importance during these two critical stages of pre- and peri- implantation development. Conclusion: Evolutionary comparison revealed that the selected subset of genes has been rewired in goat and human/goat similarity is greater than the mouse/goat or bovine/goat similarities. The developed profiles provide a resource for comprehensive understanding of goat preimplantation development and pluripotent stem cell engineering as well.
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نویسندگان مقاله پوریا حسین نیا | pouria hossein nia


مجتبی طهمورث پور | mojtaba tahmoorespur


سید مرتضی حسینی | sayyed morteza hosseini


مهدی حاجیان | mehdi hajian


سمیه ostadhosseini | somayyeh ostadhosseini


محمدرضا نصیری | mohammad reza nasiri


محمد حسین نصر اصفهانی | mohammad hossein nasr esfahani


نشانی اینترنتی http://ijfs.ir/journal/article/abstract/4659
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/72/article-72-367974.pdf
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