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International Journal of Fertility and Sterility، جلد ۱۰، شماره ۱، صفحات ۶۲-۷۰
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عنوان انگلیسی Fetal RHD Genotyping Using Real-Time Polymerase Chain Reaction Analysis of Cell-Free Fetal DNA in Pregnancy of RhD Negative Women in South of Iran
چکیده انگلیسی مقاله Objective: Maternal-fetal RhD antigen incompatibility causes approximately 50% of clinically significant alloimmunization cases. The routine use of prophylactic anti-D immunoglobulin has dramatically reduced hemolytic disease of the fetus and newborn. Recently, fetal RHD genotyping in RhD negative pregnant women has been suggested for appropriate use of anti-D immunoglobulin antenatal prophylaxis and decrease unnecessary prenatal interventions. Materials and methods: In this prospective cohort study, in order to develop a reliable and non-invasive method for fetal RHD genotyping, cell free fetal DNA (cffDNA) was extracted from maternal plasma. Real-time quantitative polymerase chain reaction (qPCR) for detection of RHD exons 7, 5, 10 and intron 4 was performed and the results were compared to the serological results of cord blood cells as the gold standard method. SRY gene and hypermethylated Ras-association domain family member 1 (RASSF1A) gene were used to confirm the presence of fetal DNA in male and female fetuses, respectively. Results: Out of 48 fetuses between 8 and 32 weeks (wks) of gestational age (GA), we correctly diagnosed 45 cases (93.75%) of RHD positive fetuses and 2 cases (4.16%) of the RHD negative one. Exon 7 was amplified in one sample, while three other RHD gene sequences were not detected; the sample was classified as inconclusive, and the RhD serology result after birth showed that the fetus was RhD-negative. Conclusion: Our results showed high accuracy of the qPCR method using cffDNA for fetal RHD genotyping and implicate on the efficiency of this technique to predict the competence of anti-D immunoglobulin administration.
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نویسندگان مقاله لیلی معزی | leili moezzi


زینب کشاورز | zeinab keshavarz


رضا رنجبران | reza ranjbaran


فرزانه aboualizadeh | farzaneh aboualizadeh


عباس بهزاد بهبهانی | abbas behzad behbahani


معصومه عبداللهی | masooma abdullahi


امین رمضانی | amin ramezani


عالم تاج صمصامی | alamtaj samsami


صدیقه شریف زاده | sedigheh sharifzadeh


نشانی اینترنتی http://ijfs.ir/journal/article/abstract/4770
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/72/article-72-368037.pdf
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