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Cell Journal، جلد ۱۲، شماره ۱، صفحات ۵۱-۵۸
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عنوان انگلیسی Bulge Cells of Rat Hair Follicles: Isolation, Cultivation, Morphological and Biological Features
چکیده انگلیسی مقاله Background: Transplants of multipotent stem cells have been shown to have a neuroprotective effect after central nervous system injury. The bulge region of the hair follicle has been reported as a putative source of hair follicle stem cells (HFSC) for many years; however, few studies have documented the properties of bulge derived cells in vitro until now. This study was conducted to isolate and culture bulge cells from rat hair follicles and to determine the morphological and biological features of the cultured cells. Materials and methods: The bulge region of the rat whisker was isolated and cultured in Dulbecco's modified eagle medium: nutrient mixture F-12 (DMEM/F12) supplemented with epidermal growth factor (EGF), cholera toxin. Dissociated bulge stem cells were differentiated on coated substrates together with NT-3. The morphological and biological features of cultured bulge cells were observed by light microscopy and immunocytochemistry methods. Results: Our results showed that newly proliferated cells could be observed on the 4th day after explantation. The expression of a neural progenitor marker, nestin, was seen before differentiation of the bulge cells. The differentiated cells expressed βIII-Tubulin and RIP, which are the markers of neural and glial lineages. Conclusion: The results indicated that the bulge cells cultured from the rat hair follicle had the characteristics of stem cells and could differentiate into neural and glial lineages.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله ملیحه نوبخت | maliheh nobakht


نوروز نجف زاده | nowruz najafzadeh


منوچهر صفری | manouchehr safari


ناهید رهبر روشندل | nahid rahbar roshandel


حمداله دلاویز | hamdollah delaviz


محمد تقی جغتایی | mohammad taghi joghataie


مهرداد بختیاری | mehrdad bakhtiyari


سارا آسالگو | sara asalgoo


فرید صفر | farid safar


نشانی اینترنتی http://celljournal.org/journal/article/abstract/3772
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/16/article-16-368095.pdf
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زبان مقاله منتشر شده en
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