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International Journal of Fertility and Sterility، جلد ۹، شماره ۲.۵، صفحات ۵۱-۵۲
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عنوان انگلیسی P-20: Efficiency of Magnetic-Activated Cell Sorting System in Separation of Normal Sperm
چکیده انگلیسی مقاله Background: Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these procedures is magnetic-activated cell sorting system (MACS) method based on surface marker. In this study, we compared sperm chromatin, protamine deficiency and morphology between density gradient centrifugation (DGC) and MACS procedures. Materials and methods: Semen samples were obtained from 20 infertile individuals with male factor. Each semen sample was divided into three fractions; first fraction was washed with PBS as unprocessed sample. The second and third fractions were processed by MACS and DGC, respectively. After each fraction, percentage of sperm DNA fragmentation, protamine deficiency and morphology were assessed using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, chromomycin A3 (CMA3) staining and papanicolaou respectively. Results: Percentages of sperm DNA fragmentation, protamine deficiency and abnormal morphology have significantly decreased after MACS and DGC procedures compared to unprocessed sample (P< 0.05). It is noticeable that MACS procedure can separate non-apoptotic sperm from apoptotic sperm population. Conclusion: Sperm selection based on surface marker (MACS) could be useful for separation of sperm with low DNA damage, low protamine deficiency, low abnormal morphology with intact acrosome and low apoptotic sperm. Therefore, we suggest that MACS procedure could be sufficient for clinical aims in the future.
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نویسندگان مقاله n زیارتی | n ziarati


m تولایی | m tavalaee


m بهادرانی | m bahadorani


mh نصر اصفهانی | mh nasr esfahani


نشانی اینترنتی http://ijfs.ir/journal/article/abstract/4372
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