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International Journal of Fertility and Sterility، جلد ۹، شماره ۲.۵، صفحات ۵۵-۵۶
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عنوان انگلیسی P-29: Antioxidants Improve The Quality of Cryopreserved SSCs
چکیده انگلیسی مقاله Background: Radiotherapy and chemotherapy could affect spermatogenesis process, so it is important to preserve fertility of patients who suffer from cancer disease. Cryopreservation of SSCs is considered as a helpful procedure for preserving male fertility in cancer patients. It seems that antioxidants can decrease ROS formation and reduce side-effects of cryopreservation on cells. In this study, we added catalase and alpha-tocopherol to the basic freezing medium in order to evaluate the effects of these two antioxidants on SSCs. Materials and methods: SSCs were isolated from testes of 3 to 6 days old mice using enzymatic digestion. The purity of isolated cells was evaluated by flow cytometry. Catalase (20 and 40 μl/ml) or alpha-tocopherol (100 and 200 μl/ml) was added to the basic freezing medium. The cell viability, ROS formation and expression of Bax and Bcl2 were evaluated in these conditions. Results: The survival rate of the freezed cells in the presence of catalase or alpha-tocopherol was significantly more than control group (P≤0.05). In addition, the Bax expression level in the catalase and alpha-tocopherol groups was significantly (P≤0.05) lower than the control group and a significant rise of Bcl2 expression was detected in the catalase and alphatocopherol groups (P≤0.05). However, the ROS production in catalase and alpha-tocopherol was significantly (P≤0.05) lower compared with the control group. Conclusion: This cryopreservation method could help to increase the number of SSCs and improve the quality and viability of these cells after cryopreservation
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نویسندگان مقاله f علی اکبری | f aliakbary


m باعزم | m baazm


h یزدخواستی | h yazdekhasti


n عباسی | n abbasi


m عباسی | m abbasi


نشانی اینترنتی http://ijfs.ir/journal/article/abstract/4381
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