این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
International Journal of Fertility and Sterility، جلد ۹، شماره ۲.۵، صفحات ۹۲-۹۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی P-119: Survey of Genetic Alterations in Exon1 of Androgen Receptor Gene in Azoospermic Patients
چکیده انگلیسی مقاله Background: Androgen receptor (AR) mediates androgen actions such as initiation and promotion of spermatogenesis and growth of accessory sex organs. There are two trinucleotide polymorphisms (CAG and GGN repeats) in exon1 of AR gene that are vary in length in population. The CAG and GGN repeats association with infertility is still unknown and this study is planned to assess the distribution of CAG and GGN repeat expansion and their roles in Iranian men’s possible infertility. Materials and methods: To achieve this aim, eighty men were chosen (40 fertile men as a control and 40 azoospermic verified using spermogram test as an infertile group). The patients genomic DNA was extracted from their peripheral blood and the AR gene was amplified by polymerase chain reaction (PCR). For determining the number of repeats, sequencing method was used. Results: Our result demonstrated the means of CAG and GGN repeat length in infertile group were 23.4 and 23.7 respectively, that were significantly higher than these trinucleotide repeats in fertile men (AR-CAG mean =21.7, P=0.014 and AR-GGN mean =22.3, P=0.001). In addition, the maximum CAG repeat in azoospermic men was 29 repeats and in control group it was 27 repeats. Longest GGN repeat in infertile men was measured 30 vs. 25 in control group. Conclusion: This finding indicates that some men with deficiency in spermatogenesis have longer trinucleotide repeats in the AR gene. According to the results, long stretches of tandem repeats of AR gene may negatively affect the function of the gene and consequently lead to male infertility in Iranian men.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله v فیروزی | v firouzi


a محسنی میبدی | a mohseni meybodi


m چهرازی | m chehrazi


h گورابی | h gourabi


ma صدیقی گیلانی | ma sadighi gilani


m صباغیان | m sabbaghian


نشانی اینترنتی http://ijfs.ir/journal/article/abstract/4474
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات