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International Journal of Fertility and Sterility، جلد ۹، شماره ۲.۵، صفحات ۲۷-۲۷
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عنوان انگلیسی O-5: Reprogramming of Paternal DNA Methylome during Spermiogenesis
چکیده انگلیسی مقاله Background: Chromatin of male and female gametes undergoes a number of reprogramming events during the transition from germ cell to embryonic developmental programs in the zygote. This process involves reorganisation of the patterns of 5-methylcytosine (5mC), a DNA modification associated with regulation of gene activity. Notably, both maternal and paternal genomes undergo Tet3-dependent oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in one-cell embryos. Although the precise biological functions of these oxidised forms of 5mC remain elusive, they may play specific roles in active demethylation and transcriptional regulation. Materials and methods: Here we present the results of genome-scale analysis of 5mC/5hmC/5caC distributions in round spermatids and spermatozoa and demonstrate that reprogramming of the paternal methylome begins during spermatid maturation. Results: We show that patterns of 5caC genomic distribution are highly dynamic during spermiogenesis. Whereas 5caC is eliminated from LINE1 retroposons and transcriptionally active spermiogenesis-specific genes during spermatid maturation, it is simultaneously accumulated at promoter regions and introns of the genes involved in embryo development. Importantly, a large fraction of 5caC-enriched genes also retain nucleosomes marked with bivalent histone modifications in spermatozoa chromatin. Conclusion: Our results suggest that embryonic patterns of DNA modifications are prearranged during spermatid maturation and imply a role for 5caC in poising the activity of developmental genes in mammalian embryogenesis.
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نویسندگان مقاله mj بلایت | mj blythe


a kocer | a kocer


jr drevet | jr drevet


a ruzov | a ruzov


نشانی اینترنتی http://ijfs.ir/journal/article/abstract/4317
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