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International Journal of Fertility and Sterility، جلد ۹، شماره ۱، صفحات ۹۳-۱۰۶
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عنوان انگلیسی Effect of Phosalone on Testicular Tissue and In Vitro Fertilizing Potential
چکیده انگلیسی مقاله Background: The current study aimed to evaluate the effects of phosalone (PLN) as an organophosphate (OP) compound on testicular tissue, hormonal alterations and embryo development in rats. Materials and methods: In this experimental study, we divided 18 mature Wistar rats into three groups-control, control-sham and test (n=6 per group). Animals in the test group received one-fourth the lethal dose (LD50) of PLN (150 mg/kg), orally, once per day for 45 days. DNA laddering and epi-fluorescent analyses were performed to evaluate testicular DNA fragmentation and RNA damage, respectively. Serum levels of testosterone and inhibin-B (IN-B) were evaluated. Testicular levels of total antioxidant capacity (TAC), total thiol molecules (TTM) and glutathione peroxidase (GSH-px) were analyzed. Finally, we estimated sperm parameters and effect of PLN on embryo development. Two-way ANOVA was used for statistical analyses. Results: There was severe DNA fragmentation and RNA damage in testicular tissue of animals that received PLN. PLN remarkably (p< 0.05) decreased testicular TAC, TTM and GSH-px levels. Animals that received PLN exhibited significantly (p< 0.05) decreased serum levels of testosterone and IN-B. Reduced sperm count, viability, motility, chromatin condensation and elevated sperm DNA damage were observed in the test group rats. PLN resulted in significant (p< 0.05) reduction of in vitro fertilizing (IVF) potential and elevated embryonic degeneration. Conclusion: PLN reduced fertilization potential and embryo development were attributed to a cascade of impacts on the testicles and sperm. PLN promoted its impact by elevating DNA and RNA damages via down-regulation of testicular endocrine activity and antioxidant status.
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نویسندگان مقاله امیر امنیت طلب | amir amniattalab


مزدک رضی | mazdak razi


نشانی اینترنتی http://ijfs.ir/journal/article/abstract/4213
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