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International Journal of Fertility and Sterility، جلد ۸، شماره ۲.۵، صفحات ۸-۸
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عنوان انگلیسی I-19: Surrogate Egg Shell Culture for The Analysis of Avian Stem Cell Fate
چکیده انگلیسی مقاله Background: The chick embryo is a classical model to study embryonic development. However, most researchers have not studied the effect of embryonic manipulation on chick hatchability. To determine the effect of egg orientation and type of sealing film on the hatchability of cultured embryos, and to determine the fate of adulte stem cells injected into the blastoderm. Materials and Methods: Windows were made in the sharp end of recipient surrogate chicken eggshells, and donor embryos were placed into the recipient eggshell for the first three days of incubation. Survival over the first three days was maximized when windowed eggs were positioned with the window-end down regardless of cling-film type. Three-day-old cultured embryos were transferred into recipient turkey eggshells, sealed with cling-film, and cultured until hatch. Avian adult stem cells expressing a reporter gene were injected into the developing embryo, and their fate was evaluated in various tissues using PCR, and immunochemistry Results: Water weight loss of the surrogate eggshell cultures regardless of cling-film types was not significantly different from control intact eggs. The embryos cultured in turkey eggshells and sealed with Handi Wrap® exhibited (p< 0.05) higher hatchability (75 ± 10.2%) than cultures sealed with Saran Wrap® (45.2 ± 13.8%). The hatchability of control intact eggs was (86.4 ± 5.3%). The hatchability of eggs sealed with Handi Wrap® was not significantly (p>0.05) different from control eggs suggesting that Handi-Wrap® was an excellent sealant for chick embryos cultured after 3 days of incubation. Conclusion: Surrogate egg shell culture is an effective way to culture avian embryos through hatching; and an effective methodology to evaluate donor stem cell fate.
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نشانی اینترنتی http://ijfs.ir/journal/article/abstract/3804
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