این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۴، شماره ۴، صفحات ۲۰۷-۲۱۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Investigation ctpA Gene Among Listeria Monocytogenes and Transferring it into E.coli
چکیده انگلیسی مقاله Introduction: Listeria monocytogenes is a Gram positive bacterium, often found in the environment and is responsible for serious food-borne diseases such as perinatal infections, septicaemia and meningoencephalitis in humans and animals. For this reason, distribution of the ctp A (copper transport protein A) among L. monocytogenes was isolated from clinical, environment, dairy, and poultry samples, and was investigated. Then, ctp A gene was transferred into E.coli DH5-?. Materials and Methods: This investigation was carried out in two steps (ctpA was found in L. monocytogenes isolated from different sources, which was kept in culture collection of Adelaide University, Australia. Then ctpA gene was transferred into E. coliDH5-?. CtpA DNA from L. monocytogenes was amplified by PCR, identified on agarose gel, purified by phenol, and ligated intopGEM-T vector. Then, it was transferred on X-gal plate containing ampicillin. The sequencing of ctpA DNA was determined by using dyeterminator kit to purify DNA, and followed by sequencing machine. Results: By using PCR to identify the homologous DNA in 69 isolates, 38% of tested isolates contained ctpA determinant. Our results showed that: 90% of clinical and dairy isolates; 85% of environmental isolates and 7% of poultry isolates of L. monocytogenes contained ctpA in chromosome DNA. Fortunately, the transformation of ctpA from L. monocytogenes into E. coli DH5-a was successful. Conclusion: Since, the existence of ctpA in clinical, dairy and environmental samples was 90% and in poultry was 7%, there fore, the virulence of all strains of this bacteria are not the same. Introducing such clone (ctpA gene) into suitable carrier strains, could be expected to produce a good oral immunogen against L. monocytogenes.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله
نشانی اینترنتی http://celljournal.org/journal/article/abstract/3562
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/16/article-16-368884.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات