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International Journal of Fertility and Sterility، جلد ۸، شماره ۲.۵، صفحات ۲۷-۲۷
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عنوان انگلیسی O-30: Comparing Expression Patterns of Endometrial Genes in Implantation Failures and Recurrent Miscarriages with Fertile Couples Following ICSI/IVF Using in Silico Analysis
چکیده انگلیسی مقاله Background: To screen and diagnose patients with recurrent abortions and implantation failure after IVF/ICSI, differentially expressed genes of endometrium through DNA microarrays were monitored. Materials and Methods: Microarray expression profile of GSE26787 dataset from GEO database was used to analyze gene expression profiles of 15 endometrial biopsy samples- five from control fertile (CF) couples, five from implantation failure (IF) and five from recurrent spontaneous miscarriage (RM), prepared by using Affymetrix Human Genome U133 Plus 2.0 system. Raw data was normalized by RMA (Robust Multichip Average) algorithm and cluster analysis was applied to compare gene expression patterns between IF and RA with CF (2 samples Bayes Test; p value < 0.05). Genes exhibiting at least 2-fold changes were selected for further analysis and also analyzed to generate a putative interaction map including proteome map based on biological processes and molecular functions of proteins. Results: The down-regulated genes in IF versus FC cases were PAEP, CXCL14, GPX3, TSPAN8 and DNER; while in RM versus IF were PDS5B, HLA-DQA1, LOC100505967, NTRK3, and PBK. Up-regulated genes in IF versus FC included LOC100505912, XDH, MMP26, LOC100505967, and CTNNA2 and in RM versus IF the up-regulated genes were GABRA2, TCN1, SLC37A2, C2CD4A, and MFSD4. Conclusion: The different expression of endometrial genes showed the extent of changes within gene expression of endometrium prior to conception that plays an important role in infertile couples especially in patients who have IF or RM. We will further analyze the findings to generate a putative interaction map including proteome map based on biological processes and molecular functions of proteins.
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نشانی اینترنتی http://ijfs.ir/journal/article/abstract/3860
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