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International Journal of Fertility and Sterility، جلد ۷، شماره ۳، صفحات ۷۸-۷۸
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عنوان انگلیسی P-107: Vitrification and In Vitro Culture of Mouse Preantral Follicles in Presence of Growth Factors
چکیده انگلیسی مقاله Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrification in vitro. Materials and Methods: Preantral follicles with diameter of 150-180 μm were mechanically isolated from 18- 21 day old NMRI mouse ovaries. Follicles were vitrified and warmed and then cultured in α-minimal essenstial medium supplemented with 0,20 ng/ml FGF,20 ng/ml HGF, 20 ng/ml from each of FGF and EGF. After 12 days hCG/EGF was added to culture medium and after 18- 20 h later the presence of cumulus oocyte complexes (COC) and oocyte maturation state assessed. Results: The results of this study showed that the rate of MII oocytes in FGF group increased in comparison with control and other treatment groups (p< 0.05) There was significant increase in survival rate of follicles in FGF-HGF group in comparison with control and other treatment groups (p< 0.05 ). After in vitro ovulation induction the follicles in HGF group showed a lower ovulation rate (p< 0.05) than those cultured in other groups. Conclusion: FGF in mouse vitrified follicle culture system increases oocyte maturation rate and HGF decreases COCs number. Addition of HGF and FGF simultaneously to the culture medium increase follicle survival.
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نشانی اینترنتی http://ijfs.ir/journal/article/abstract/3568
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