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International Journal of Fertility and Sterility، جلد ۷، شماره ۳، صفحات ۸۴-۸۴
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عنوان انگلیسی P-124: Association between Fertilization Rate and Externilization of Phosphatidyl Serine
چکیده انگلیسی مقاله Background: Externalization of PS (EPS) is considered as a sign of early apoptosis. In addition, EPS also occurs during capacitation and the acrosome reaction. During this study we aimed to evaluate the relationship between EPS and fertilization, embryo quality and pregnancy outcomes in couples who were candidates for ICSI. Materials and Methods: Semen samples (n = 43) were collected from infertile individuals ICSI candidates and assessed according to World Health Organization guidelines for semen parameters. EPS was assessed by Annexin V and propidium iodide (PI) staining. Results: There is a significant positive correlation between percentages of fertilization and annexin-positive PI-negative (An+PI-) sperm and percentage of An+ PIsperm was significantly high in individuals with fertilization rates higher than 50%. In addition, the percentage of annexin-positive PI-positive (An+PI+) sperm in semen of the partners of pregnant women significantly differed from the partners of non-pregnant women. Furthermore, a significant negative correlation between percentages of fertilization and protamine-deficient sperm was observed. Conclusion: The result of this study suggest that An+PIis a sign of capacitation and An+PI+ is a sign of apoptosis, therefore, semen samples with high percentage of An+PI-, have a higher ability to undergo capacitation and, also have a higher chance to result in successful fertilization post-ICSI.
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نشانی اینترنتی http://ijfs.ir/journal/article/abstract/3585
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