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International Journal of Fertility and Sterility، جلد ۳، شماره ۴، صفحات ۱۶۵-۱۷۰
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عنوان انگلیسی Evaluation of Embryos Derived from in vitro Fertilized Oocytes Reconstructed by Meiosis-II Chromosome Transplantation from Aged Mice to Ooplasms of Young Mice
چکیده انگلیسی مقاله Background: To assess embryos derived by the transfer of meiosis-II chromosomes (M-II-t) from aged mice oocytes into ooplasms from younger mice to overcome the problem of age-related decline in female fertility. Materials and methods: The developmental capacity karyotype and ultrastructure of reconstructed oocytes derived from meiosis-II chromosome transplantation from aged mice into the ooplasms of young mice by piezo-micromanipulation were assessed. Results: The survival rate of enucleated young oocytes was 54% and the percent of fertilized reconstructed oocytes was 23%. The rate of embryo development to the two-cell stage after cultivation was 40%. Since 82.4% of the analyzed embryos derived from reconstructed oocytes had condensed nuclei it was not possible to analyze their chromosomal integrity. However 17.6% of analyzable reconstructed old oocyte derived embryos (old-ODEs) had normal diploid sets of chromosomes. Major structural differences were not observed between young old and M-II-t derived two-cell embryos. Conclusion: Our findings suggested that ooplasms from younger mice may overcome ageassociated problems in older mice.
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نویسندگان مقاله عبدالحسین شاهوردی | abdolhossein shahverdi


منصوره موحدین | mansoureh movahedin


مجتبی رضازاده ولوجردی | mojtaba rezazadeh valojerdi


حسین بهاروند | hossein baharvand


نشانی اینترنتی http://ijfs.ir/journal/article/abstract/2380
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/72/article-72-370106.pdf
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