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Persian Journal of Acarology، جلد ۶، شماره ۲، صفحات ۰-۰
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عنوان انگلیسی Multiplex-PCR differentiation of two Hyalomma and two Haemaphysalis species (Acari: Ixodidae)
چکیده انگلیسی مقاله Hyalomma and Haemaphysalis are two most important genera of ticks (Acari: Ixodidae). The rapid and accurate identification of field collected specimens is crucial in faunal works, laboratory assays, anti-tick vaccine experiments, etc. The present study was designed to introduce a rapid and more sensitive method for the differentiation of closely related Hyalomma and Haemaphysalis species namely the pairs Hy. anatolicum - Hy. asiaticum and Ha. punctata-Ha. sulcata , as the main vectors of different animal and human pathogenic agents. Tick specimens were collected from domestic animals in Ardabil, Hormozgan, Khuzestan, Kurdistan, Lorestan, and Mazandaran and identified according to the taxonomic keys. DNA was extracted by the Phenol-Chloroform method. Then, PCR was carried out in a single PCR reaction tube using three pairs designed primers (one forward and two reverse) adapted from the internal transcribe spacer 2 (ITS2) and cytochrome oxidase subunit 1 (COI) genes for Hyalomma and Haemaphysalis , respectively. In the present study, results of Multiplex-PCR revealed that the pairs Hy. anatolicum - H. asiaticum and Ha. punctata-Ha. sulcata could be well differentiated on gel electrophoresis. Morphological misidentification of Hy. anatolicum - Hy. asiaticum and Ha. punctata-Ha. sulcata could be reduced significantly after using Multiplex-PCR.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله اسدالله حسینی چگنی | asadollah hosseini chegeni
razi herbal medicines research center, lorestan university of medical sciences, khorramabad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی لرستان (Lorestan university of medical sciences)

محمد حسن کایدی | mohammad hassan kayedi
department of parasitology and mycology, school of medicine, lorestan university of medical sciences, lorestan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی لرستان (Lorestan university of medical sciences)

رضا حسینی | reza hosseini
department of plant protection, faculty of agriculture, university of guilan, rasht

سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)

زکیه تلمادره ای | zakkyeh telmadarraiy
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://biotaxa.org/pja/article/view/24392
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/528/article-528-371409.pdf
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زبان مقاله منتشر شده en
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