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Iranian Journal of Ichthyology، جلد ۲، شماره ۳، صفحات ۱۷۲-۱۷۶
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عنوان انگلیسی Polymorphism of insulin-like growth factor binding protein-3 (IGFBP-3) gene associated with growth traits in Cyprinus carpio
چکیده انگلیسی مقاله The genetic polymorphism of insulin-like growth factor binding protein ( IGFBP-3 ) and its association with the growth traits namely condition factor, body weight, and total length were investigated in common carp Cyprinus carpio using PCR- single strand conformation polymorphism (SSCP) technique. Caudal fin clips from 120 C. carpio , aging 5 months, were used in this study and a 330 bp DNA fragment was amplified from 5'-UTR of carp IGFBP-3 . SSCP genotyping yielded seven different banding patterns namely A, B, C, D, E, F, and G with frequencies of 14.16, 12.5, 21.67, 9.17, 11.67, 10.0, and 20.84, respectively. The obtained results showed a relatively high polymorphic behavior of 5'-UTR regulatory region of studied locus; therefore it shows the importance of this region in regulation of transcription of IGFBP-3 gene in carp. Marker-trait association showed significant relationships between the detected genotypes and condition factor (CF). IGFBP-3 gene polymorphism in joint with CF signifies relatively high genetic diversity and significant potential for marker-assisted selection in this species.
کلیدواژه‌های انگلیسی مقاله Common carp, Genetic variations, Growth traits, IGFBP-3.
نویسندگان مقاله فرزانه محرابی | farzaneh mehrabi
laboratory of molecular genetics and animal biotechnology, department of animal sciences, college of animal science and fisheries, sanru, sari, iran.


محمد کاظم خالصی | mohammad kazem khalesi
department of fisheries, faculty of animal science and fisheries , sari university of agricultural sciences and natural resources sanru , sari, iran


ایوب فرهادی | ayoub farhadi
laboratory of molecular genetics and animal biotechnology, department of animal sciences, college of animal science and fisheries, sanru, sari, iran.
نشانی اینترنتی http://ijichthyol.org/index.php/iji/article/view/43
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/451/article-451-383417.pdf
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