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International Journal of Horticultural Science and Technology، جلد ۱، شماره ۱، صفحات ۳۱-۴۱
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عنوان انگلیسی Agrobacterium-Mediated Transformation of Pomegranate (Punica granatum L.) ‘Yousef Khani’ Using the gus Reporter Gene
چکیده انگلیسی مقاله In this study, an efficient Agrobacterium-mediated transformation method was developed forpomegranate (Punica granatum L.), a difficult-to-transform plant. In vitro shoot segments wereinoculated with Agrobacterium tumefaciens strain LBA4404 harboring the binary vectorpBI121 carrying the neomycin phosphotransferase (nptII) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter. After 28 d in WPM selection medium containing 50 mgL-1 kanamycin, 59 new shoots proliferated. gus analysis was performed on these putativetransgenic shoots, of which 32 stained positive. Positive staining shoots were cut and culturedin selection medium for 2 subsequent subcultures until final gus analysis. After three months ofthe selection period, 6 putative transgenic shoots were obtained. Presence of the gus and nptIIgenes was confirmed by polymerase chain reaction. Southern blot analysis confirmed that TDNAwas stably integrated into the genome of three out of six PCR-positive plants. Thetransgenic plants were rooted and successfully acclimatized.
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نویسندگان مقاله بابک ولی زاده کاجی | valizadeh kaji
department of horticultural science, faculty of agriculture, bu-ali sina university, azadegan boulevard, hamedan, iran
سازمان اصلی تایید شده: دانشگاه بوعلی سینا (Bu ali sina university)

احمد ارشادی |
department of horticultural science, faculty of agriculture, bu-ali sina university, azadegan boulevard, hamedan, iran
سازمان اصلی تایید شده: دانشگاه بوعلی سینا (Bu ali sina university)

مسعود توحیدفر |
agricultural biotechnology research institute of iran abrii , mahdasht road, karaj, iran

نشانی اینترنتی http://ijhst.ut.ac.ir/article_35097_df18c25cf5ff7e268a571483bce7b505.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/413/article-413-385704.pdf
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