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Journal of Arthropod-Borne Diseases، جلد ۱۰، شماره ۴، صفحات ۴۴۵-۰
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عنوان انگلیسی Serological and Molecular Detection of Dirofilaria Species in Stray Dogs and Investigation of Wolbachia DNA by PCR in Turkey
چکیده انگلیسی مقاله Background: Dirofilaria immitis and Dirofilaria repens are the most common species of filarial nematodes described in the dogs. A single-step multiplex PCR was applied to detect and differentiate simultaneously and unequivocally D. im­mitis and D. repens on DNA extracted from canine peripheral blood and besides to detect the seroprevalance of D. immitis by ELISA in Elazig Province, Turkey. A PCR detection of the Wolbachia , which plays an important role in D. immitis biology and contributes to the inflammatory pathology of the heartworm, was also applied for the first time in Turkey. Methods: A total of 161 whole blood and sera samples were collected from stray dogs and stored at -20 °C until used. After DNA extraction, all samples were processed with Dirofilaria primers by multiplex-PCR and Wolbachia primers by conventional PCR besides ELISA for serology. The amplification was performed using a set of primers designed on a portion of the small subunit ribosomal RNA gene of the mitochondrion (12S rDNA). Results: Three of the examined dogs (1.8%) were found to be infected with only D. immitis , one (0.6%) with D. repens and three (1.8%) with both parasites. Besides, 10 out of 161 dogs (6.2%) were found infected with Wolbachia sp. Finaly, the seroprevalence of dirofilariosis in the examined dogs was found to be 3.7% (6/161). Conclusion: Although dirofilariosis is not a serious problem in the region, the stray dogs still continue to be a source of infection.
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نویسندگان مقاله سامی simsek | sami simsek
department of parasitology, faculty of veterinary medicine university of firat, elazig, turkey


آیسه ترکان ciftci | ayse turkan ciftci
parasitology and bee diseases laboratory, veterinary control and research institute, elazig, turkey
نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/324
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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