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Journal of Arthropod-Borne Diseases، جلد ۱۰، شماره ۴، صفحات ۵۱۹-۰
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عنوان انگلیسی Isolation and Purification of an Antibacterial Protein from Immune Induced Haemolymph of American Cockroach, Periplaneta americana
چکیده انگلیسی مقاله Background: Antimicrobial peptides play a role as effectors substances in the immunity of vertebrate and inverte­brate hosts. In the current study, antimicrobial peptide was isolated from the haemolymph of the American cock­roach, Periplaneta americana . Methods: Micrococcus luteus as Gram-positive bacteria and Escherichia coli as Gram-negative bacteria were candi­date for injection. Induction was done by injecting both bacteria into the abdominal cavity of two groups of cock­roaches separately. The haemolymphs were collected 24 hours after post injection and initially tested against both bacteria. Subsequently, the immune induced haemolymph was purified by high performance liquid chromatography (HPLC) to separate the proteins responsible for the antibacterial activity. Results: The non-induced haemolymph did not show any activity against both bacteria whereas induced haemo­lymph exhibited high activity against M. luteus but did less against E. coli . Two fractions showed antibacterial activ­ity against M. luteus. F inally the molecular weight of the isolated antibacterial proteins were determined as 72 kDa and 62 kDa using SDS-PAGE. Conclusion: Induced haemolymph of American cockroaches has the ability to produce peptides to combat against Gram-positive bacteria when an immune challenge is mounted. Further work has to be done to sequence of the pro­tein, which it would be advantageous.
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نویسندگان مقاله حمید رضا باصری | hamid reza basseri
department of medical entomology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

امیر دادی خویینی | amir dadi khoeni
department of biology, payame noor university, tehran, iran

سازمان اصلی تایید شده: دانشگاه پیام نور تهران (Payame noor university)

روناک بختیاری | ronak bakhtiari
departmet of photobiology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

ماندانا ابوالحسنی | mandan abolhassani
department of medical entomology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

رضا حاجی حسینی baghdadabadi | reza hajihosseini baghdadabadi
department of biology, payame noor university, tehran, iran

سازمان اصلی تایید شده: دانشگاه پیام نور تهران (Payame noor university)

نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/337
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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