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Journal of Arthropod-Borne Diseases، جلد ۹، شماره ۲، صفحات ۱۷۶-۱۸۳
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عنوان انگلیسی Antigenic Cross-Reactivity Anti-Birtoxin Antibody against Androctonus crassicauda Venom
چکیده انگلیسی مقاله Background : Antivenom is still widely used in the treatment of envenomation as there are no vaccines or other effective agents available against animal venoms. Recently, neurotoxins named birtoxin family have been described from Parabuthus transvaalicus and Androctonus crassicauda. The aim of the present study was to test the antibirtoxin antibodies for their ability to neutralize the lethal effects of A. crassicauda scorpion venom. Methods : SDS-PAGE and Western blotting used the presence of components from A. crassicauda and P.transvaalicus scorpion venoms and to determine the degree of cross-reactivity. The Minimum Lethal Dose (MLD) of venom was assessed by subcutaneously (sc) injections in mice. Results: The MLD of the A. crassicauda venom was 35 μg/ 20g mouse by sc injection route. Western blotting showed the presence of components from A. crassicauda and P. transvaalicus scorpion venoms strongly cross react with the A. crassicauda antivenom. However, Western blotting of the A. crassicauda scorpion venom using the Refik Saydam Public Health Agency (RSPHA) generated antibody showed that not all the venom components cross reacted with the anti-birtoxin antibody. The antibodies only cross reacted with components falling under the 19 kDa protein size of A. crassicauda venom. Conclusion : The bioassays and Western blotting of A. crassicauda venom with the anti-birtoxin antibodies produced against a synthetic peptide showed that these antibodies cross reacted but did not neutralize the venom of A. crassicauda.
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نویسندگان مقاله suhandanadigüzel وان zoelen | suhandanadigüzel van zoelen
netherlands organisation for applied scientific research-innovation for life-hollanda, turkey.


ozcan ozkan | ozcan ozkan
drug and medical device agency of turkey, ankara, turkey.


بورا inceoglu | bora inceoglu
department of entomology, university of california, davis, usa.
نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/140
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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