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Journal of Arthropod-Borne Diseases، جلد ۵، شماره ۲، صفحات ۶۹-۷۶
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عنوان انگلیسی Production and Purification of Anti-Rhombomys opimus Immunoglobulins
چکیده انگلیسی مقاله Background: Zoonotic cutaneous leishmaniasis (ZCL) is an increasing public health problem in some endemic re­gions. Horseradish peroxidase (HRP) conjugated rabbit anti- Rhombomys opimus ( R. opimus ) Ig is needed for im­munoblotting and ELISA tests used to explore the immune response of the rodents against the sand fly saliva. In this study, the production of HRP conjugated rabbit anti- R. opimus Ig was conducted for the first time. Methods: Rhombomys opimus Ig was purified from serum by protein G affinity chromatography column and in­jected into rabbit to produce anti- R. opimus Ig antibody. The titration of antibody against R. opimus Ig in rabbit se­rum was checked using indirect ELISA. Rabbit anti- R. opimus Ig was purified by Sepharose-4B- R. opimus Ig column. Re­activity of this antibody was assessed by indirect ELISA and was conjugated to HRP by periodate method. Results: Approximately 3.5 mg Ig was purified from 1 ml R. opimus serum using protein G affinity chromatography col­umn. The molecular weight of purified R. opimus Ig was estimated about 150 kDa by SDS-PAGE. Nearly 2.3 mg rab­bit anti- R. opimus Ig was purified from 1 ml immunized rabbit serum. The purified antibody was conjugated to HRP and the optimum titer of HRP conjugated rabbit anti- R. opimus Ig was determined as 1:8000 using direct ELISA. Conclusion: HRP conjugated rabbit anti-Gerbil IgG has been produced by a few companies, but to our knowledge HRP conjugated rabbit anti- R. opimus Ig is not commercially available. Production of HRP conjugated rabbit anti- R. opimus Ig is considerably helpful for immunological studies of R. opimus , the main reservoir host of ZCL in Iran as well as some other countries.
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نویسندگان مقاله aa اخوان | aa akhavan
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

r قدس | r ghods
department of immunochemistry, monoclonal antibody research center, avicenna research institute, acecr, tehran, iran


m جدی تهرانی | m jeddi tehrani
department of hybridoma, monoclonal antibody research center, avicenna research institute, acecr, tehran, iran


mr یعقوبی ارشادی | mr yaghoobi ershadi
department of medical entomology and vector control, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

a خامسی پور | a khamesipour
center for research and training in skin diseases and leprosy, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

ar محمودی | ar mahmoudi
department of immunochemistry, monoclonal antibody research center, avicenna research institute, acecr, tehran, iran
نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/93
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