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Journal of Arthropod-Borne Diseases، جلد ۵، شماره ۱، صفحات ۱۳-۱۹
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عنوان انگلیسی The Effectiveness of Trypsin Modulating Oostatic Factor (TMOF) and Combination of TMOF with Bacillus thuringiensis Against Aedes aegypti Larvae in the Laboratory
چکیده انگلیسی مقاله Background: Trypsin Modulating Oostatic Factor (TMOF) terminates trypsin biosynthesis in adult and larval mos­quito gut. It will inhibit the growth and development of mosquito larvae feeding on it resulting in death by starvation. The objective of this study is to determine the effective dose of Pichia-TMOF and the combination of Pichia-TMOF with Bacillus thuringiensi s ( Bt) as larvicide on Ae. aegypti larvae. Methods: Aedes aegypti first-instar larvae were exposed to various doses of Pichia-TMOF, Bt and combination of Pichia-TMOF and Bt. The development of the larvae were observed and recorded daily during the bioassay larval test until the adult emergence. Results: The results showed that 400 ppm Pichia-TMOF and 300 ppm Pichia-TMOF were able to cause 100% and 67% cumulative mortality on Ae. aegypti larvae on 8 th day respectively. At 200 ppm, 100 ppm and 50 ppm con­centra­tion Pichia-TMOF showed obvious stunted effect on Ae. aegypti larvae. Moreover, the combination of 400 ppm Pichia-TMOF with 0.1 ppm Bt showed synergistic effect on Ae. aegypti. Conclusion : Pichia-TMOF inhibited trypsin biosynthesis is potential to act in larval gut causing stunted growth and lar­val development and causing mortality. The combination of Pichia-TMOF and Bt increased the effectiveness in caus­ing larval mortality.
کلیدواژه‌های انگلیسی مقاله Pichia-TMOF, Bt combination, Aedes aegypti larvae
نویسندگان مقاله ys lau | ys lau
department of biomedical science, faculty of allied health sciences universiti kebangsaan malaysia jalan raja muda abdul aziz 50300 kuala lumpur, malaysia


s سلیمان | s sulaiman
department of biomedical science, faculty of allied health sciences universiti kebangsaan malaysia jalan raja muda abdul aziz 50300 kuala lumpur, malaysia


h احمد عثمان | h othman
department of biomedical science, faculty of allied health sciences universiti kebangsaan malaysia jalan raja muda abdul aziz 50300 kuala lumpur, malaysia
نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/79
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