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Journal of Arthropod-Borne Diseases، جلد ۳، شماره ۲، صفحات ۲۲-۳۵

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عنوان انگلیسی Identification of Sand flies of the Subgenus Larroussius based on Molecular and Morphological Characters in North Western Iran
چکیده انگلیسی مقاله Background: The adult female sand flies (Diptera: Psychodidae) of the subgenus Larroussius are important vectors of Leishmania infantum (Kinetoplastida: Tripanosomatidae) in Meshkinshahr district, Northwest of Iran. Four Phle­boto­mus ( Larroussius ) species are present in this area, i.e. Phlebotomus ( Larroussius ) kandelakii , P. ( La. ) major , P. ( La. ) perfiliewi and P. ( La. ) tobbi . The objective of the present study was to identify and distinguish the females of P. per­filiewi , P. major and P. tobbi , in this district. Methods: Adult sand flies were collected with sticky papers, CDC light traps, and aspirator in 2006. Individual sand flies of this four species from thirty different locations were characterized morphologically and by comparative DNA se­quences analyses of a fragment of mitochondrial gene Cytochrome b (Cyt b) and nuclear gene Elongation Factor 1- al­pha (EF-1α). PCR amplification was carried out for all three species P. major , P. perfiliewi and P. tobbi in the sub­ge­nus Larroussius . Results: Phylogenetic analyses of P. major populations in this study displayed two different populations and genetic di­ver­sity. Spermathecal segment number, pharyngeal armature and other morphological characters of these three spe­cies were examined and found to present consistent interspecific differences. Conclusion: According to our findings, the phylogeny of Cyt b and EF-1α haplotypes confirms the relationships be­tween P. major , P. tobbi and P. perfiliewi as already defined by their morphological similarities.
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نویسندگان مقاله a آب سواران | a absavaran
departement of medical entomology and vector control, school of public health, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

راثی | y rassi
departement of medical entomology and vector control, school of public health, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

p پرویزی | p parvizi
department of parasitology, institute of pasteur, tehran, iran


ma عشاقی | ma oshaghi
departement of medical entomology and vector control, school of public health, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

mr عبایی | mr abaie
departement of medical entomology and vector control, school of public health, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

s رفیع زاده | s rafizadeh
center of disease control, tehran, iran


m محبعلی | m mohebali
department of parasitology, school of public health, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

z زارع | z zarea
institute of public health research, meshkinshahr health research station, iran


دکتر عزت الدین | e javadian
departement of medical entomology and vector control, school of public health, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)


نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/52
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زبان مقاله منتشر شده en
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