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Journal of Arthropod-Borne Diseases، جلد ۱، شماره ۱، صفحات ۳۸-۴۲
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عنوان انگلیسی The Seminested PCR Based Detection of Leishmania infantum Infection in Asymptomatic Dogs in a New Endemic Focus of Visceral Leishmaniasis in Iran
چکیده انگلیسی مقاله Visceral Leishmaniasis (Kala-azar) is a serious health problem in some northern and south western parts of Iran. The incidence of kala-azar caused by Leishmania infantum has recently increased in Nourabad-Mamassani district of Fars Province, in the south of the country. This study was designed to determine the role of asymptomatic dogs as host reservoir of L. infantum in this new formed focus and detection of prevalence of infection near them. A total of 20 as‌ymptomatic stray and sheep dogs were randomly sampled. The Buffy coat layer of their peripheral blood was used for DNA extraction and PCR. A species specific seminested PCR was used for DNA amplification using LINR4, LIN17 and LIN19 primers. These primers amplified variable area of the minicircle kDNA of Leishmania parasites. Of the 20 sampled dogs checked for leishmanial kDNA, six (30%) were found naturally infected. It is concluded that, dogs (Canis familiaris) even if asympto‌matic, is considered as the domestic host reservoir of kala-azar in this endemic focus.
کلیدواژه‌های انگلیسی مقاله Leishmania infantum, Dogs, Iran
نویسندگان مقاله راثی | y rassi
dept. of medical entomology and vector control, school of public health and institute of health research, medical sciences tehran university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

دکتر کوروش | k azizi
dept. of medical entomology and vector control, school of public health and institute of health research, medical sciences tehran university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

mh معتضدیان | mh motazedian
dept. of medical parasitology, shiraz university of medical sciences, shiraz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

دکتر عزت الدین | e javadian
dept. of medical entomology and vector control, school of public health and institute of health research, medical sciences tehran university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

s رفیع زاده | s rafizadeh
center of disease control cdc , genetic office, tehran, iran.


m فخار | m fakhar
dept. of medical parasitology, shiraz university of medical sciences, shiraz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

gr حاتم | gr hatam
dept. of medical parasitology, shiraz university of medical sciences, shiraz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/7
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