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Iranian Journal of Biotechnology، جلد ۱۵، شماره ۱، صفحات ۲۲-۳۲
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عنوان انگلیسی Computational Identification of Micro RNAs and Their Targets in Field Mustard (Brassica rapa L.)
چکیده انگلیسی مقاله Background: MicroRNAs (miRNAs) are a class of non-protein coding endogenous small RNAs that regulate the genes involved in plant growth and development as well as response to environmental stresses post-transcriptionally. Objective: In the present study, we report results of a systemic search for potential homologs of Brassica rapa miRNAs deposited in the miRNA Registry Database using EST analysis.Materials and Methods: Plant mature miRNA sequences were searched in non-protein coding ESTs registered in NCBI EST database. The secondary structures of the potent EST sequences were generated using Zuker folding algorithm. Potential sequences were candidate as miRNA genes and characterized evolutionarily only and if only they fit the some described criteria. Also, the web tool psRNATarget was applied to predict candidate B. rapa miRNA targets.Results: : In this study, 10 novel miRNAs from B. rapa belonging to 6 miRNA families with appropriate fold back structures were identified using EST-based homology analysis by considering a series of filtration criteria. Several potential targets with known/unkonwn functions for these novel miRNAs were identified. The target genes mainly encode transcription factors, enzymes, DNA binding proteins, disease resistance proteins, carrier proteins and other biological processes.Conclusions: MicroRNA having diverse functions in plant species growth, development and evolution by post-transcriptionally regulating the levels of specific transcriptome so by effecting on their translation products. Research in miRNA led to the identification of many miRNAs and their regulating genes from various plant species.
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نویسندگان مقاله naser farrokhi |
department of biotechnology engineering, faculty of energy engineering and new technologies shahid beheshti university
سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

بهزاد حاجی اقراری |
department of bioinformatics, institute of biochemistry and biophysics ibb , university of tehran, tehran, iran
سازمان اصلی تایید شده: مرکز تحقیقات بیوشیمی و بیوفیزیک (Institute of biochemistry and biophysics)

بهرام گلیایی |
departments of biophysics and bioinformatics laboratories, institute of biochemistry and biophysics ibb , university of tehran, tehran, iran
سازمان اصلی تایید شده: مرکز تحقیقات بیوشیمی و بیوفیزیک (Institute of biochemistry and biophysics)

کاوه کاوسی |
laboratory of complex biological systems and bioinformatics cbb , institute of biochemistry and biophysics ibb , university of tehran, tehran, iran
سازمان اصلی تایید شده: مرکز تحقیقات بیوشیمی و بیوفیزیک (Institute of biochemistry and biophysics)

نشانی اینترنتی http://www.ijbiotech.com/article_44057_4782d0c1c5b5011f9f15cfda8e128119.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/441/article-441-400705.pdf
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