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Research in Pharmaceutical Sciences، جلد ۱۲، شماره ۳، صفحات ۲۱۱-۲۲۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The allele frequency of CYP2C9 and VKORC1 in the Southern Khorasan population
چکیده انگلیسی مقاله The genetic factors are determinants in required dosage changes of warfarin among which are polymorphisms of CYP2C9 and VKORC1 genes. The present study aimed to determine the allele and genotype frequency of CYP2C9 and VKORC1 genes in Birjand population. This study was conducted on 120 individuals who referred to Imam Reza and Vali-Asr hospitals for PT/INR test. After extracting the genomic DNA, the considered sequences were amplified by PCR, and restriction fragment length polymorphism analysis was done by AvaII and KpnI enzymes to determine allele polymorphisms. Moreover, related sequences of VKORC1 , after amplification, were sequenced for determining the genotype. Allelic and genotypic frequencies as well as Hardy-Weinberg equilibrium, observed heterozygosity, expected heterozygosity, and polymorphism information content were calculated by PowerMarker V 3.25 software. Amongst 120 individuals in this study with the mean age of 58.12 ± 12.7 years, 80.8%, 9.1%, and 10% exhibited the alleles of 1, 2, and 3 CYP2C9 gene, respectively. The genotype frequencies of 1/1, 1/2, 2/2, 3/1, 3/2, and 3/3 of this gene were found to be 64.1, 15.8, 0, 17.5, 2.5, and 0 %, respectively. In -1639 G>A region, VKORC1 had normal homozygote genotype (GG) and in 1173 C>T region, heterozygote (CT) with the frequency of 48.7% and 45.9% had the most prevalence. Compared with other populations, there is a considerable difference between the allele frequency of CYP2C9 and VKORC1 genetic variance. Since 35.8% of the selected populations carry an abnormal allele causing sensitivity to warfarin, the specialists at medical centers must be informed about the genotypes of patients before prescribing warfarin.
کلیدواژه‌های انگلیسی مقاله Warfarin,Coagulant,CYP2C9,VKORC1,Polymorphism
نویسندگان مقاله فریبا عمادیان رضوی | fariba emadian razavi
1department of prosthodontics, faculty of dentistry, birjand university of medical sciences, birjand, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بیرجند (Birjand university of medical sciences)

اصغر زربان | asghar zarban
2department of biochemistry, faculty of medicine, birjand university of medical sciences, birjand, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بیرجند (Birjand university of medical sciences)

فاطمه حاجی پور | fatemeh hajipoor
3genomics research group, cellular and molecular research center, birjand university of medical sciences, birjand, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بیرجند (Birjand university of medical sciences)

محسن ناصری | mohsen naseri
4genomics research group, cellular and molecular research center, paramedical faculty, birjand university of medical sciences, birjand, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بیرجند (Birjand university of medical sciences)

نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/1763
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/115/article-115-402746.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
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