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Acta Medica Iranica، جلد ۵۵، شماره ۶، صفحات ۳۶۰-۳۶۷
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عنوان انگلیسی A Practical Non-Extraction Direct Liquid Chromatography Method for Determination of Thiopurine S-Methyltransferase Activity in Inflammatory Bowel Disease
چکیده انگلیسی مقاله Thiopurine drugs remain pivotal therapies for the wide varieties of diseases such as inflammatory bowel disease (IBD). Here, thiopurine S-methyltransferase (TPMT) phenotype, the main metabolizing enzyme of thiopurine-drugs, was studied. This is for the first time that TPMT activity is measured in Iranian IBD patients. We used an improved direct liquid chromatography assay without need for solvent extraction and minimize excess labor handling making it ideal for use in routine referral medical centers. TPMT activity in whole blood was determined by a non-extraction HPLC method. We evaluated 427 individuals including 215 IBD patients and 212 unrelated healthy individuals as control group from Iran’s western population. TPMT phenotyping of this study demonstrated no frequency for deficient, 2.8 % for low and 97.2% for normal activity, which is different with results of other studies. There was a significant negative correlation between TPMT activities as calculated based on nmol/grHb/h and the Hb-levels in IBD and control groups (r= -0.54, P < 0.001 and r= -0.27, P < 0.001), respectively. Interestingly a significant positive correlation between Hb levels and TPMT-activities were seen when the activity calculated in mU/L in IBD patients and control subjects (r=0.14, P =0.05 and r=0.43, P < 0.001), respectively. We strongly suggest the use of international unit (mU/L) is more appropriate than nmol6MTG/grHb/h for expressing TPMT-activity in IBD patients. In addition, in comparison with other providers of TPMT test activity and centers around the world the risk of toxicity is much lower after utilizing thiopurine drugs for IBD patients in this region.
کلیدواژه‌های انگلیسی مقاله HPLC,IBD,Phenotypes,Thiopurine drugs,TPMT
نویسندگان مقاله فریبرز بهره مند | fariborz bahrehmand
fertility and infertility research center, kermanshah university of medical sciences, kermanshah, iran. and department of clinical biochemistry, school of medicine, kermanshah university of medical sciences, kermanshah, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمانشاه (Kermanshah university of medical sciences)

امیر کیانی | amir kiani
regenerative medicine research center, school of medicine, kermanshah university of medical sciences, kermanshah, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمانشاه (Kermanshah university of medical sciences)

اسد ویسی رایگانی | asad vaisi raygani
fertility and infertility research center, kermanshah university of medical sciences, kermanshah, iran. and department of clinical biochemistry, school of medicine, kermanshah university of medical sciences, kermanshah, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمانشاه (Kermanshah university of medical sciences)

همایون بشیری | homayoun bashiri
department of internal medicine, kermanshah university of medical sciences, kermanshah, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمانشاه (Kermanshah university of medical sciences)

مهدی زبیری | mehdi zobeiri
department of internal medicine, kermanshah university of medical sciences, kermanshah, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمانشاه (Kermanshah university of medical sciences)

علی معینی | ali moini
department of internal medicine, kermanshah university of medical sciences, kermanshah, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمانشاه (Kermanshah university of medical sciences)

طیبه پورمتعبد | tayebeh pourmotabbed
department of microbiology, immunology and biochemistry, university of tennessee health science center, memphis, tn, usa.
نشانی اینترنتی http://acta.tums.ac.ir/index.php/acta/article/view/5962
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/56/article-56-421413.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Articles
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