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Research in Pharmaceutical Sciences، جلد ۱۲، شماره ۴، صفحات ۳۰۷-۳۱۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Method development and validation of HPLC tandem/mass spectrometry for quantification of perindopril arginine and amlodipine besylate combination in bulk and pharmaceutical formulations
چکیده انگلیسی مقاله A well-characterized and fully validated ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometric (UHPLC-ESI-MS/MS) method was developed to reliably analyze combination of perindopril arginine and amlodipine besylate in bulk and tablet formulations. The chromatographic separation was achieved on a Waters ACQUITY UPLC ® BEH C18 column with 1.7 μm particle packing which enabled the higher peak capacity, greater resolution, increased sensitivity, and higher speed of analysis using a volatile mobile phase ideally being at least 2 pH units below and above the perindopril arginine and amlodipine besylate pKa, respectively. Mass spectrometric detection was performed using electrospray ion source in positive ion polarity to profile the abundances of perindopril arginine and amlodipine besylate, using the transitions m/z 369 → m/z 172, and m/z 409 → m/z 238 for perindopril arginine and amlodipine besylate, respectively. Calibration curve was constructed over the range 0.25 – 500 ng/mL and 1.0 – 100 ng/mL for perindopril arginine and amlodipine besylate, respectively. The method was precise and accurate, and provided recovery rates > 80% for both compounds. Furthermore, the intra- and inter-assay precision in terms of % RSD was in between 0.1 – 3.7 for both perindopril arginine and amlodipine besylate. A specific, accurate, and precise UHPLC-MS/MS method for the determination of perindopril arginine and amlodipine besylate in bulk and tablet formulation.
کلیدواژه‌های انگلیسی مقاله Tandem mass spectrometry,Perindopril,Amlodipine
نویسندگان مقاله kalaiyarasi duraisamy | kalaiyarasi duraisamy
1analytical research and development, dr. reddy’s laboratories ltd, hyderabad, india. 2department of pharmaceutical sciences, jawaharlal nehru technological university, hyderabad, india.


ks jaganathan | ks jaganathan
3analytical research and development, shantha biotechnics limited, hyderabad, india.


marothu vamsi krishna | marothu vamsi krishna
4department of pharmaceutical analysis, alliance institute of advanced pharmaceutical and health sciences, hyderabad, india.
نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/1774
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/115/article-115-421900.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
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