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Iranian Journal of Toxicology، جلد ۱۱، شماره ۵، صفحات ۲۳-۳۰
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عنوان انگلیسی Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro Experiment
چکیده انگلیسی مقاله Background: The global incidence of snakebite has become a major concern to the community. This study aimed to evaluate the effect of mango seed kernel methanol extract on metalloproteases in Carpet Viper (Echis ocellatus) venom. Methods: Mango seed kernel methanolic extract was evaluated in vitro for its anti-venom activity and inhibition of metalloproteases of Carpet Viper's (Echis ocellatus) venom. Metalloprotease portion was partially purified from the venom of E. ocellatus with a yield of 71%, a purification fold of 2.63 and a specific activity of 19.00 µmol/min/mg protein. Results: The enzyme appeared as a band on SDS-PAGE with a molecular weight of 23 kDa. The kinetic properties of the enzyme showed a Km of 0.31 mg mL-1 and a Vmax of 9.09 µmol min-1. When the enzyme was incubated with the extract, kinetic studies revealed a mixed non-competitive pattern of inhibition with Km values of 0.56 and 1.11 mg mL-1 and Vmaxvalues of 6.67 and 4.17-µmol min-1 for 5% and 20% inhibitor concentrations, respectively. An estimated Ki value of 0.168 mg mL-1 was obtained from a secondary plot demonstrating that the extract had a high affinity for the partially purified enzyme; thus, could serve as an effective inhibitor. Conclusion: Methanol extract of mango seed kernel has a high affinity for the partially purified enzyme, and it might provide an inexpensive and readily available alternative to sheep serum in the management of snakebite envenomation. Therefore, further in vivo studies are necessary to assess its effectiveness and safety.
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نویسندگان مقاله peculiar nwanyibunwa okoro | peculiar nwanyibunwa okoro
department of basic research, national research institute for chemical technology, zaria, nigeria.


ثانی ابراهیم | sani ibrahim
department of biochemistry, ahmadu bello university, zaria, nigeria.


hajiya mairo inuwa | hajiya mairo inuwa
department of biochemistry, ahmadu bello university, zaria, nigeria.


stanley irobekhian reuben okoduwa | stanley irobekhian reuben okoduwa
department of research and development, nigerian institute of leather and science technology, zaria, nigeria.
نشانی اینترنتی http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-416-1&slc_lang=en&sid=en
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/138/article-138-423017.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده عمومی
نوع مقاله منتشر شده پژوهشی
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