| چکیده انگلیسی مقاله |
Background : 5-Fluorouracil could lead to a decline in fertility in Caenorhabditis elegans. The aim of this study was to describe the mechanisms underlying such an altered fertility phenotype and to illustrate the specific genes and path-ways that are involved in the related phenotypic changes in C. elegans. Methods : We isolated total RNA from the samples and used a new method called Digital Gene Expression (DGE), which can rapidly identify genes with altered transcript levels. The random genes were confirmed by real-time RT-PCR. Results : We analyzed the results of two methods to draw conclusions based on a comparison between C. elegans and other harmful parasites. Compared with controls, 1147 genes were up-regulated, and 1067 were down-regulated. Overall, 101 up-regulated genes had a log2 ratio higher than 8, whereas the log2 ratio of 141 down-regulated genes was higher than 8. After mapping to the reference database, 4 pathways were confirmed to be involved in this phe-nomenon, with statistically significant participation from 19 genes. Conclusion : For the first time, the transcript sequence of 5-Fu-treated worms and controls was detected. We found that 4 possible pathways, i.e., ECM-receptor interaction pathway, TGF-beta signaling pathway, Focal adhesion and Hypertrophic cardiomyopathy, may be involved in the number decline in the embryos of C. elegans. Specifically, the ECM-receptor interaction pathway and Focal adhesion may be very important pathways that alter the reproduction of C. elegans. |
| نویسندگان مقاله |
si زانگ | si zhang
pathogens biology institute, school of basic medical sciences, lanzhou university, gansu, china and maternal and child health hospital of fengtai district of beijing city, beijing, china.
zhaolian lu | zhaolian lu
pathogens biology institute, school of basic medical sciences, lanzhou university, gansu, china.
hongmei liang | hongmei liang
pathogens biology institute, school of basic medical sciences, lanzhou university, gansu, china.
ximei fu | ximei fu
pathogens biology institute, school of basic medical sciences, lanzhou university, gansu, china.
یان زانگ | yan zhang
dept. of anesthesiology, first hospital, lanzhou university, gansu, china.
xin liu | xin liu
institute of integrated chinese and western medicine, school of basic medical sciences, lanzhou university, gansu, china.
genshu bao | genshu bao
pathogens biology institute, school of basic medical sciences, lanzhou university, gansu, china
تایو جینگ | tao jing
pathogens biology institute, school of basic medical sciences, lanzhou university, gansu, china
xuan ونگ | xuan wang
pathogens biology institute, school of basic medical sciences, lanzhou university, gansu, china and suqian hospital of traditional chinese medicine, jiangsu, china.
meng ونگ | meng wang
pathogens biology institute, school of basic medical sciences, lanzhou university, gansu, china and tianjin first center hospital, tianjin, china.
julong wu | julong wu
pathogens biology institute, school of basic medical sciences, lanzhou university, gansu, china and shandong center for disease control and prevention, shandong, china.
gen chen | gen chen
pathogens biology institute, school of basic medical sciences, lanzhou university, gansu, china and key lab of preclinical study for new drugs of gansu province, gansu, china.
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