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Iranian Journal of Public Health، جلد ۴۴، شماره ۸، صفحات ۱۱۱۴-۱۱۲۰
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عنوان انگلیسی Rocket and Two Dimensional Immunoelectrophoresis in Diag-nosis of Caprine Brucellosis
چکیده انگلیسی مقاله Background : Brucellosis is a major bacterial zoonosis of global importance with the causative organisms of Gram-negative facultative intracellular pathogens. The aims of this study were to standardize two immunoelectrophoretic techniques, rocket and cross immunoelectrophoresis, and compare their results with other conventional serodiagnostic tests. Methods : Sera from 15 sheep, without any history of brucellosis vaccination, infected with Brucella melitensis M16 sub-cutaneously, were employed in a comparison of culture, precipitating, and immunoelectrophoretic tests. A 125 days serologic follow-up was performed after the infection was started. As a reference, these tests also done in the five healthy sheep. Results : The results obtained with the rocket immunoelectrophoresis test correlated very well with those of the cross immunoelectrophoresis, whereas results of other tests such as culture, Rose Bengal, standard tube agglutination and 2-mercaptoethanol seruagglutination tests were inferior. Conclusion : As agglutination test shows cross reaction and a prozone phenomenon, and in blood culture, the bacte-ria is not always detectable, so they are time consuming rocket and cross immunoelectrophoresis are recommended because their results can be obtained in a shorter time.
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نویسندگان مقاله داود مهربانی | davood mehrabani
stem cell and transgenic technology research center, dept. of pathology, shiraz university of medical sciences, shiraz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

زهرا غلامی | zahra gholami
dept. of bacteriology and virology, school of medicine, shiraz university of medical sciences, shiraz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

جمشید کهن طب | jamshid kohanteb
dept. of bacteriology and virology, school of medicine, shiraz university of medical sciences, shiraz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

مسعود سپهری منش | masood sepehrimanesh
gasteroenterohepatology research center, shiraz university of medical sciences, shiraz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

سید محمد حسین حسینی | seyedmohammad hossein hosseini
razi vaccine and serum research institute, shiraz, iran.

سازمان اصلی تایید شده: موسسه تحقیقات واکسن و سرم سازی رازی (Razi vaccine and serum research institute)

نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/3361
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زبان مقاله منتشر شده en
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