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Iranian Journal of Biotechnology، جلد ۱۵، شماره ۳، صفحات ۱۶۶-۱۷۱
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عنوان انگلیسی Stimulation of Camel Polyclonal Antibody against Human T cell Immunoglobulin and Mucin 3
چکیده انگلیسی مقاله Background: T cell Immunoglobulin, Mucin (TIM)-3, is a type I transmembrane glycoprotein belonging to TIM family. This receptor expresses on T helper type 1 (Th1) cells that binds to galectin-9 (Gal9); inducing an inhibitory signal. As a result, apoptosis of Th1 cells occurs and cytotoxicity of CD8 T cells becomes evident in vitro. Therefore, this immunomodulatory molecule may be used as a novel target for clinical purposes. The production of camel polyclonal antibodies against TIM-3-expressing cell line was the purpose of this study. Objectives: In this study, we aimed to use HEK 293 cells expressing human TIM-3 to obtain camel polyclonal antibody against TIM-3 by immunization. Materials and Methods: A pre-synthesized human TIM-3cDNA was inserted into pcDNA3.1 plasmid and the new construct was transfected in HEK cell. TIM-3 expression was confirmed by qRT-PCR and flow cytometry. A camel (6 months old) was immunized with the lysate prepared from rTIM-3 expressing HEK cells 4 times. The anti-TIM-3 antibody level was evaluated using ELISA method. Results: TIM-3 was successfully cloned in HEK cells with 88% success rate. High level of anti-TIM-3 antibody was detected in the serum of the camel immunized with the recombinant cell lysate, after final injection. Conclusions: Our rhTIM-3 cell display system can be useful for future diagnostic or therapeutic approaches.
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نویسندگان مقاله ویدا همایونی |
department of immunology, faculty of medicine, isfahan university of medical sciences, isfahan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

حسین خان احمد |
department of genetic, faculty of medicine, isfahan university of medical sciences. isfahan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

مزدک گنجعلی خانی حاکمی | ganjalikhani hakemi
department of immunology, faculty of medicine, isfahan university of medical sciences, isfahan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

مهدی بهدانی |
biotechnology research center, biotechnology department, venom amp; biotherapeutics molecules lab, pasteur institute of iran, tehran, iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

پوریا قاسمی |
department of immunology, faculty of medicine, isfahan university of medical sciences, isfahan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

عباس رضایی |
department of immunology, faculty of medicine, isfahan university of medical sciences, isfahan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

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