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Iranian Journal of Biotechnology، جلد ۱۵، شماره ۳، صفحات ۱۸۶-۱۹۳
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Expression Profiling of Hspb1 and Tp53 Genes through RT-qPCR in Different Cancer Types of Canis familiaris
چکیده انگلیسی مقاله Background: Diagnostic molecular marker studies are in vogue to have insight of most prevalent animal diseases including cancer. Objectives: Gene expression profiling of pro and anti-apoptotic genes was conducted in dog Lymphoma, CTVT, SCC, granuloma, perianal adenocarcinoma and mammary tumors. Materials and Methods: Cancerous tissues of 21 affected animals were obtained. Total RNA was extracted followed by cDNA synthesis. Comparative Ct method via Taqman assay (RT-qPCR) was used to quantify corresponding mRNA molecules, Tp53 and Hspb1, as normalized by GAPDH as the reference gene . Results: Hspb1 showedectopic expression in lymphoma, CTVT and mammary tumors; its down-regulation was observed in granuloma and oral SCC with fold difference (FD) of ±35. Similarly, Tp53 as the tumor suppressor gene with pro-apoptotic properties, showed up-regulation in all tumor types, notably 80% of mammary tumors and 60% of CTVT. The FD values were 33.31 and 2.27, respectively. Conclusion: Altered transcriptomic response of Hspb1 and Tp53 was observed in all cancer types of Canis familiaris. The resulting profile depicts the involvement of the genes in cancer pathways. Thus, the data might be helpful for diagnosis, prognosis, identification and classification of these widespread neoplasms in this species.
کلیدواژه‌های انگلیسی مقاله Dog neoplasias,Hspb1,Livak method,RT-qPCR,TaqMan probes,Tp53
نویسندگان مقاله رشید سیف |
department of biotechnology, virtual university of pakistan, lahore 54000, pakistan

علیرضا awan | ali raza
institute of biochemistry and biotechnology, university of veterinary and animal sciences, outfall road, lahore 54000, pakistan

محمد طیب |
institute of biochemistry and biotechnology, university of veterinary and animal sciences, outfall road, lahore 54000, pakistan

مسرور الهی babar | ellahi babar
department of biotechnology, virtual university of pakistan, lahore 54000, pakistan

آسیم خالید محمود | khalid mahmood
pet center, university of veterinary and animal sciences, outfall road, 5400, lahore, pakistan

ضیای اله |
pet center, university of veterinary and animal sciences, outfall road, 5400, lahore, pakistan

سعیدا ضیای |
department of sciences and humanities, national university of computer and emerging sciences, lahore, pakistan

محمد wasim |
institute of biochemistry and biotechnology, university of veterinary and animal sciences, outfall road, lahore 54000, pakistan

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