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Iranian Journal of Public Health، جلد ۴۲، شماره ۱۱، صفحات ۱۲۴۲-۵۲
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عنوان انگلیسی Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap.
چکیده انگلیسی مقاله TGIFLX, a Homoproteins cluster member located on the X chromosome, has a critical role in male reproduction and prostate development. Previous studies have shown the erratic expression of TGIFLX gene in a large proportion of prostate tumors. However TGIFLX function in prostate development remains unknown. The purpose of this study was to evaluate the consequences of TGIFLX expression on prostate cancer cell lines (LNCaP).Inducible Tet-On gene expression system was used with a regulatory capability by doxycycline induction. In this system, stable LNCaP cells with TGIFLX tet-on plasmid were able to induce TGIFLX expression by doxycycline treatment. TGIFLX gene expression was confirmed by RT-PCR.Induction of gene expression caused cell proliferation decrement and apoptosis increment in LNCaP TGIFLX cells compared with control cells (P< 0.01). Also, by using PEGFPN1 plasmid protein in this study localization was shown in nucleus. The gene was cloned in the plasmid and transfected to LNcap cells with plasmid PEGFPN1 TGIFLX and the plasmid was PEGFPN1. The TGIFLX expression was confirmed by RT-PCR and fluorescent microscopy.TGIFLX expression demonstrated a tumor suppressor characterization in a prostatic cancer cell line with low grade of tumorigenicity (LNCaP). More cell lines with different level of tumorogenicity need to be investigated for further clarification of the TGIFLX gene function.
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نویسندگان مقاله زهرا رشوند | zahra rashvand
1. dept. of medical genetics, tehran university of medical sciences tums , tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

منصور حیدری | mansour heidari
1. dept. of medical genetics, tehran university of medical sciences tums , tehran, iran ; 2. stem cell preparation unit, farabi eye hospital, tehran university of medical sciences , tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

رضا روفیان | reza raoofian
1. dept. of medical genetics, tehran university of medical sciences tums , tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمد حسین مدرسی | mohammad hossein modarresi
1. dept. of medical genetics, tehran university of medical sciences tums , tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

رضا شیرکوهی | reza shirkoohi
3. group of genetics, cancer research center, cancer institute of iran, tehran university of medical sciences tums , tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/4356
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