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Iranian Journal of Public Health، جلد ۴۱، شماره ۸، صفحات ۸۴-۸۸
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عنوان انگلیسی Identification of Prototheca zopfii from Bovine Mastitis
چکیده انگلیسی مقاله Background: The aim of this study was identification of the epidemiology of Prototheca zopfii species from the milk samples of dairy cattle in Isfahan, central Iran. Methods: Milk samples were obtained from 230 dairy cattle, 130 with and 100 without mastitis, in Isfahan. The samples were cultured in Prototheca Isolation Medium (PIM) and Sabouraud's dextrose agar. All P. zopfii isolates were identified by morphological and biochemical methods. Then, as a confirmatory test they were examined by genotype-specific PCR. Results: Four P. zopfii strains (3.07%) were isolated from the 130 samples of dairy cattle with clinical mastitis and there was no isolation from totally 100 samples of healthy bovines without mastitis. Specific PCR product (about 946 bp) was detected in four isolates. Conclusion: It seems that P. zopfii genotype II plays a key role in affecting bovine mastitis that confirmed other previous studies. Our study was the first, which identified the Prototheca species by traditional and molecular methods in Iran and Middle East as well.
کلیدواژه‌های انگلیسی مقاله Bovine mastitis, Prototheca zopfii, Iran
نویسندگان مقاله زینی | f zaini


a کنعانی | a kanani


فلاحتی | m falahati


r فاتح | r fateh


m سلیمی اصل | m salimi asl


n صایمی | n saemi


sh فره یار | sh farahyar


a کارگر خیرآباد | a kargar kheirabad


ناظری | m nazeri


نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/2537
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