این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Public Health، جلد ۴۰، شماره ۴، صفحات ۱۳۸-۱۴۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Detection of Borrelia persica Infection in Ornithodoros tholo¬zani Using PCR Targeting rrs Gene and Xenodiagnosis
چکیده انگلیسی مقاله Background: Relapsing fever caused by Borrelia persica , is an acute tick-borne disease which is transmitted by soft ticks of Ornithodoros tholozani to human. Methods: Value of PCR and xenodiagnosis for detection of B. persica in O. tholozani ticks was compared. Sixty-four Bor­relia -free ticks were fed on infected guinea pigs and used for the experiments. For xenodiagnosis, a group of 32 ticks in subsequent blood meal were fed on sterile guinea pigs and the indication of B. persica in the animal blood was tested 5-14 days later by dark-field microscopy. For PCR, all 64 ticks were subjected to PCR against B. persica rrs gene (16S-rDNA). Also sensitivity of PCR in terms of minimum detectable number of spirochetes as well as the effects of tick sex and post digestion was tested. Results: PCR revealed B.persica DNA in 98.4% ticks, in which B. persica were found in 25.0% by xenodiagnosis. PCR was enough sensitive to give positive results for DNA of 1 spirochete. PCR success rates were similar for male or female ticks. Course of time did not affect the efficacy of PCR and similar results were observed for ticks of immediately fed, semi- or completely gravid or completely digested blood ones. Conclusion: Our results indicate that due to very low specificity and time consuming, xenodiagnosis is not a useful method whereas PCR method has advantages for study the Borrelia prevalence in ticks.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله رفیع نژاد | j rafinejad
dept. of medical entomology and vector control, school of public health, tehran university of medica

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

n چوبدار | n choubdar
dept. of medical entomology and vector control, school of public health, tehran university of medica

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

ma عشاقی | ma oshaghi
dept. of medical entomology and vector control, school of public health, tehran university of medica

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

n پیازک | n piazak
dept. of parasitology, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

t سطوت | t satvat
dept. of medical parasitology, school of public health, tehran university of medical sciences, tehra

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

f محترمی | f mohtarami
dept. of medical entomology and vector control, school of public health, tehran university of medica

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

a برمکی | a barmaki
dept. of medical entomology and vector control, school of public health, tehran university of medica

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/3023
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات