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Iranian Journal of Public Health، جلد ۳۹، شماره ۴، صفحات ۶۴-۶۹
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عنوان انگلیسی Evaluation of a Single PCR Assays on Cp5 Gene for Differentiation of Entamoeba histolytica and E. dispar
چکیده انگلیسی مقاله Background: We examined a molecular method with a single-PCR for amplification of a part of CP5 gene enabling us to differen­tiate the pathogenic species, Entamoeba histolytica , from the non-pathogenic species, E. dispar . Methods: We developed a single PCR method for this purpose. After investigation of GenBank, primer pairs were de­signed from highly conserved regions of cysteine proteinase (CP5) gene. The primers were utilized in PCR using isolated ge­nomic DNA template of E. histolytica and the PCR products were then sequenced. The same primer and method for PCR was used for isolated genomic DNA template of E. dispar . Results: A fragment of about 950 bp was isolated in PCR by using DNA from E. histolytica , however, no banding pattern was produced by using the same primers for E. dispar . We characterized CP5 gene at molecular level in E. histolytica iso­lates from 22 positive; including 20 non-dysentery samples isolated from both cities as well as two dysentery samples iso­lated only from Tabriz. Nucleotide sequence comparison in gene data banks (NCBI, NIH) revealed significant homology with CP5 gene in E. histolytica isolates Conclusion: We developed a PCR method, which could detect simply and rapidly E. histolytica by amplifying a specific PCR fragment.
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نویسندگان مقاله s rostamighalehjaghi | s rostamighalehjaghi


r جمالی | r jamali


رضایی | s rezaie


z بابایی | z babaei


h هوشیار | h hooshyar


محمد رضاییان | m rezaeian


نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/3082
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