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Iranian Journal of Public Health، جلد ۳۸، شماره ۲، صفحات ۲۷-۳۶
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عنوان انگلیسی Evaluating of VDR Gene Variation and its Interaction with Immune Regulatory Molecules in Osteoporosis
چکیده انگلیسی مقاله Background: To evaluate VDR gene variation and its interaction with immune regulatory molecules in osteoporosis. Methods: Totally 205 pre and postmenopausal women were recruited in the study. After an overnight fast, peripheral blood was taken and centrifuged to sprat serum for measurement of serum parathyroid hormone, 25 hydroxyvitamin D, osteocal­cin and cross laps. The Fok I polymorphism in exon 2 of the VDR gene was detected by PCR-RFLP. Expression of osteopro­tegrin, vitamin D receptor ( VDR ) and β-actin genes were quantified by quantitative real-time reverse transcriptase. To design the experimental model we randomly selected five participants of each genotype groups. PBMC were cultured and induced with vitamin D. At several times, cells were harvested and total RNA was extracted .Then expression of target genes evaluated by real time PCR. Results: The frequencies of Ff, FF and ff genotypes were 34.2%, 56.5% and 9.2%. The mean of bone mineral density in FF genotype was higher than other genotypes. Also in this genotype, mean of serum inflammatory cytokines was lower than other genotypes. The expressions of the VDR and osteoprotegrin were up regulated by 1, 25(OH) 2 D 3 in PBMC from participants with FF genotype. PBMC from healthy control comparison to osteoporotic patients had a clearly better response to vitamin D 3 incubation. Conclusion: Inflammation may important role in osteoporosis whereas osteoporotic patients have elevated pro-inflamma­tory profile. This cytokine profile and gene expressions of VDR and Osteoprotegrin were different in VDR genotype groups.
کلیدواژه‌های انگلیسی مقاله Vitamin D receptor, Polymorphism, Cytokines, Gene expression
نویسندگان مقاله a حسین نژاد | a hossein nezhad
national institute of genetic engineering and bio technology, tehran, iran


gh آهنگری | gh ahangari
national institute of genetic engineering and bio technology, tehran, iran


b لاریجانی | b larijani
endocrinology and metabolism research center, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/3186
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